Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production

doi:10.1016/j.biortech.2018.08.007

	Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production
	Song, Xiaojin 1; Wang, Jie 3; Wang, Yanchao 1; Feng, Yingang 1; Cui, Qiu 1; Lu, Yandu 2
刊名	BIORESOURCE TECHNOLOGY
	2018-11
卷号	268 页码:340-345
关键词	Chlorella Functional food Heavy-ion irradiation mutagenesis Mutant creation Sweet sorghum juice
ISSN号	0960-8524
DOI	10.1016/j.biortech.2018.08.007
英文摘要	To improve the economic viability of Chlorella as feedstock for food commodities, a serial of concentrations of low-cost sweet sorghum juice (SSJ), alternative to glucose, were used for the fermentation of Chlorella pyrenoidosa. A high biomass and protein production (8.91 g L (-1) biomass and 4.52 g L (-1) protein) was revealed with 20% SSJ. To further increase productivity, heavy-ion irradiation-mediated mutagenesis was employed to create mutants where a strain K05, with desired phenotypes (increased biomass and protein production in pilot-scale fermentation), was screened. Compared with the parental strain, the production of biomass, proteins, and chlorophylls of mutant K05 increased by 11.6%, 31.8%, and 7.6%, respectively. Production capacities under industrial scale (two-ton) further pinpoint the stability and scalability of mutant K05. These results suggest that advances in cultivation techniques coupled with artificial strain improvement will further promote microalgae as an attractive platform of functional food.
资助项目	General Financial Grant from the China Postdoctoral Science Foundation[2016M602211]
WOS关键词	SWEET SORGHUM JUICE ; LIPID PRODUCTIVITY ; HETEROTROPHIC CULTIVATION ; MIXOTROPHIC CULTIVATION ; HEAVY-IONS ; NANNOCHLOROPSIS ; ACID ; OPTIMIZATION ; MUTAGENESIS ; IMPROVEMENT
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000445043600043
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/59662]
专题	中国科学院近代物理研究所
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, CAS Key Lab Biofuels, Qingdao Engn Lab Single Cell Oil,Qingdao Inst Bio, Qingdao 266101, Shandong, Peoples R China 2.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Coll Oceanol, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Song, Xiaojin,Wang, Jie,Wang, Yanchao,et al. Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production[J]. BIORESOURCE TECHNOLOGY,2018,268:340-345.
APA	Song, Xiaojin,Wang, Jie,Wang, Yanchao,Feng, Yingang,Cui, Qiu,&Lu, Yandu.(2018).Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production.BIORESOURCE TECHNOLOGY,268,340-345.
MLA	Song, Xiaojin,et al."Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production".BIORESOURCE TECHNOLOGY 268(2018):340-345.