Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi:10.1038/srep35131

	Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling
	Zhang, Baoping 1,2,3; Li, Long 1,3; Li, Zhiqiang 4; Liu, Yang2 ; Zhang, Hong2 ; Wang, Jizeng 1,3
刊名	SCIENTIFIC REPORTS
	2016-10-12
卷号	6 页码:13
ISSN号	2045-2322
DOI	10.1038/srep35131
英文摘要	A apoptotic model was established based on the results of five hepatocellular carcinoma cell (HCC) lines irradiated with carbon ions to investigate the coupling interplay between apoptotic signaling and morphological and mechanical cellular remodeling. The expression levels of key apoptotic proteins and the changes in morphological characteristics and mechanical properties were systematically examined in the irradiated HCC lines. We observed that caspase-3 was activated and that the Bax/Bcl-2 ratio was significantly increased over time. Cellular morphology and mechanics analyses indicated monotonic decreases in spatial sizes, an increase in surface roughness, a considerable reduction in stiffness, and disassembly of the cytoskeletal architecture. A theoretical model of apoptosis revealed that mechanical changes in cells induce the characteristic cellular budding of apoptotic bodies. Statistical analysis indicated that the projected area, stiffness, and cytoskeletal density of the irradiated cells were positively correlated, whereas stiffness and caspase-3 expression were negatively correlated, suggesting a tight coupling interplay between the cellular structures, mechanical properties, and apoptotic protein levels. These results help to clarify a novel arbitration mechanism of cellular demise induced by carbon ions. This biomechanics strategy for evaluating apoptosis contributes to our understanding of cancer-killing mechanisms in the context of carbon ion radiotherapy.
资助项目	National Natural Science Foundation of China[11032006] ; National Natural Science Foundation of China[11121202] ; National Natural Science Foundation of China[11472119] ; National Natural Science Foundation of China[11421062] ; National Natural Science Foundation of China[11305224] ; National Natural Science Foundation of China[11575262]
WOS关键词	RELATIVE BIOLOGICAL EFFECTIVENESS ; CYTOSKELETAL DISRUPTION ; ACTIN CYTOSKELETON ; CASPASE ACTIVATION ; DEATH ; CANCER ; GELSOLIN ; BEAMS ; MECHANOTRANSDUCTION ; AUTOPHAGY
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000384991500001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43628]
专题	中国科学院近代物理研究所
通讯作者	Liu, Yang; Wang, Jizeng
作者单位	1.Lanzhou Univ, Coll Civil Engn & Mech, Key Lab Mech Disaster & Environm Western China, Minist Educ, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Radiat Med, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, Inst Biomech & Med Engn, Lanzhou 730000, Peoples R China 4.Northwest Univ Nationalities, Key Lab Oral Dis Gansu Prov, Lanzhou 730030, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Baoping,Li, Long,Li, Zhiqiang,et al. Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling[J]. SCIENTIFIC REPORTS,2016,6:13.
APA	Zhang, Baoping,Li, Long,Li, Zhiqiang,Liu, Yang,Zhang, Hong,&Wang, Jizeng.(2016).Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling.SCIENTIFIC REPORTS,6,13.
MLA	Zhang, Baoping,et al."Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling".SCIENTIFIC REPORTS 6(2016):13.