Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions

doi:10.1016/j.jsb.2007.09.016

	Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions
	Liu, Hongrong 1; Cheng, Lingpeng 2,3; Zeng, Songjun 1; Cai, Canying 1; Zhou, Z. Hong 2,3; Yang, Qibin 1
刊名	JOURNAL OF STRUCTURAL BIOLOGY
	2008
卷号	161 页码:64-73
关键词	cryo-electron microscopy virus structure three-dimensional reconstruction icosahedral symmetry-adapted functions
ISSN号	1047-8477
DOI	10.1016/j.jsb.2007.09.016
英文摘要	Three-dimensional (3D) reconstruction is the last and an essential step toward high-resolution structural determination in single-particle cryo-electron microscopy (cryoEM). We have implemented a new algorithm for reconstructing 3D structures of macromolecular complexes with icosahedral symmetry from cryoEM images. Icosahedral symmetry-adapted functions (ISAFs) are used to interpolate structural factors in the reciprocal space to generate a 3D reconstruction in spherical coordinates. In our implementation, we introduced a recursive method for deriving higher order ISAFs from three lower order seed functions. We demonstrate improvements of our new method in both the noise suppression and the effective resolution in 3D reconstruction over the commonly used Fourier-Bessel synthesis method introduced by Crowther et al. three decades ago. Our 3D reconstruction method can be extended to macromolecular complexes with other symmetry types and is thus likely to impact future high-resolution cryoEM single-particle reconstruction efforts in general. (c) 2007 Elsevier Inc. All rights reserved.
WOS关键词	ELECTRON CRYOMICROSCOPY ; 3-DIMENSIONAL RECONSTRUCTION ; CRYOELECTRON MICROSCOPY ; ICOSAHEDRAL GROUP ; ANGSTROM RESOLUTION ; DISORDERED SPECIMENS ; VIRUS STRUCTURES ; MICROGRAPHS ; PROTEIN ; MODEL
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000252228200007
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/28250]
专题	中国科学院近代物理研究所
通讯作者	Yang, Qibin
作者单位	1.Xiangtan Univ, Inst Modern Phys, Xiangtan 411105, Hunan, Peoples R China 2.Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA 3.Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Liu, Hongrong,Cheng, Lingpeng,Zeng, Songjun,et al. Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions[J]. JOURNAL OF STRUCTURAL BIOLOGY,2008,161:64-73.
APA	Liu, Hongrong,Cheng, Lingpeng,Zeng, Songjun,Cai, Canying,Zhou, Z. Hong,&Yang, Qibin.(2008).Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions.JOURNAL OF STRUCTURAL BIOLOGY,161,64-73.
MLA	Liu, Hongrong,et al."Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions".JOURNAL OF STRUCTURAL BIOLOGY 161(2008):64-73.