Ginsenoside re promotes human sperm capacitation through nitric oxide-dependent pathway

doi:10.1002/mrd.20583

	Ginsenoside re promotes human sperm capacitation through nitric oxide-dependent pathway
	Zhang, Hong; Zhou, Qingming; Li, Xiaoda; Zhao, Weiping; Wang, Yanling; Liu, Hui; Li, Ning
刊名	MOLECULAR REPRODUCTION AND DEVELOPMENT
	2007-04-01
卷号	74 页码:497-501
关键词	human sperm capacitation acrosome reaction Ginsenoside Re nitric oxide cGMP cGMP-dependent protein kinase
ISSN号	1040-452X
DOI	10.1002/mrd.20583
英文摘要	The regulation of sperm capacitation is important for successful fertilization. Ginsenosides, the biologically effective components of ginseng, have been found to enhance intracellular nitric oxide (NO) production and the latter has recently been indicated to play a significant role in modulation of sperm functions. We investigated the effect of Ginsenoside Re on human sperm capacitation in vitro and the mechanism by which the Ginsenosides play their roles. Spermatozoa were separated by Percoll and incubated with 0, 1, 10, or 100 mu M of Ginsenoside Re. The percentages of spontaneous and lysophosphatidylcholine (LPC)-induced acrosome reaction (AR), as a measure of sperm capacitation, were assayed with fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA). The intracellular cGMP level was measured by [H-3] cGMP radioimmunoassay system. The results showed that the percentages of both spontaneous and LPC-induced AR and intracellular cGMP level were significantly enhanced by Ginsenoside Re with a concentration-dependent manner. Sodium nitroprusside (SNP, 100 nM), a NO donor, mimicked the effects of Ginsenoside Re. And pretreatment with a NOS inhibitor N-omega-nitro-L-arginine methyl ester (L-NAME, 100 mu M) or a NO scavenger N-acetyl-L-cysteine (LNAC, 1 mM) completely blocked the effects of Ginsenoside Re. Furthermore, the AR-inducing effect of Ginsenoside Re was significantly reduced in the presence of the soluble guanylate cyclase inhibitor LY83583 or cGMP-dependent protein kinase (PCK) inhibitor KT5823, whereas addition of the cGMP analogue 8-Br-cGMP significantly increased the AR of human spermatozoa. Data suggested that Ginsenoside Re is beneficial to sperm capacitation and AR, and that the effect is accomplished through NO/cGMP/PKG pathway.
WOS关键词	CRYOPRESERVED BOVINE SPERMATOZOA ; ACROSOME REACTION ; IN-VITRO ; LIPID-PEROXIDATION ; FOLLICULAR-FLUID ; SYNTHASE ; MOTILITY ; INDUCTION ; CONTRACTION ; EXOCYTOSIS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
语种	英语
出版者	WILEY-LISS
WOS记录号	WOS:000244253500012
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/27165]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hong,Zhou, Qingming,Li, Xiaoda,et al. Ginsenoside re promotes human sperm capacitation through nitric oxide-dependent pathway[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2007,74:497-501.
APA	Zhang, Hong.,Zhou, Qingming.,Li, Xiaoda.,Zhao, Weiping.,Wang, Yanling.,...&Li, Ning.(2007).Ginsenoside re promotes human sperm capacitation through nitric oxide-dependent pathway.MOLECULAR REPRODUCTION AND DEVELOPMENT,74,497-501.
MLA	Zhang, Hong,et al."Ginsenoside re promotes human sperm capacitation through nitric oxide-dependent pathway".MOLECULAR REPRODUCTION AND DEVELOPMENT 74(2007):497-501.