Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD

doi:10.2174/0929866525666180122145846

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD
	Zhang, Wei 1; Yu, Jigang 1; Zhang, Xuecheng 1; Peng, Hui 1; Li, Xinxin 1; Zhang, Jiahai 2; Sun, Hongbin 3; Tu, Xiaoming 2
刊名	PROTEIN AND PEPTIDE LETTERS
	2018
卷号	25 期号:4 页码:362-367
关键词	Starch binding domain molten globule ligand induced folding carbohydrate binding module family 69 AmyP nuclear magnetic resonance
ISSN号	0929-8665
DOI	10.2174/0929866525666180122145846
通讯作者	Zhang, Xuecheng(turenzh@ahu.edu.cn) ; Peng, Hui(pph0259@126.com)
英文摘要	AmyP is an alpha-amylase which shows preferential degradation to soluble starch. In this substrate preference its Starch Binding Domain (SBD), which was recently assigned to a new Carbohydrate Binding Module (CBM) family 69, plays an important role. In the present study, the SBD of AmyP (AmyP-SBD) was recombinantly expressed, purified, and structurally characterized. Using Circular Dichroism (CD), intrinsic fluorescence, and nuclear magnetic resonance (NMR) spectroscopy, the structures of AmyP-SBD in the absence and presence of substrate analogue beta-cyclodextrin were measured. The results intriguingly showed that free form AmyP-SBD is partially unfolded, like a compact molten globule, and could be induced by the ligand to fold into a relatively rigid state. Further structure determination for folded AmyP-SBD revealed a topology distinctive from those of SBDs from other CBM families. Our data indicate AmyP-SBD is a structurally novel SBD, and this may be helpful for understanding the properties of AmyP-SBD and CBM69 and elucidation of functioning mechanism of AmyP.
资助项目	National Natural Science Foundation of China[31470775] ; Key Research Program of the Education Department of Anhui Province[KJ2014ZD01] ; International Science and Technology Cooperation Plan of Anhui Province[1503062010]
WOS关键词	ASPERGILLUS-NIGER GLUCOAMYLASE ; ALPHA-AMYLASE ENZYMES ; MOLTEN GLOBULE ; NMR ; HYDROLYSIS ; EVOLUTION ; PROTEINS ; GTPASE ; SYSTEM ; FAMILY
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000435213800006
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; Key Research Program of the Education Department of Anhui Province ; 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内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/38185]
专题	合肥物质科学研究院_中科院强磁场科学中心
通讯作者	Zhang, Xuecheng; Peng, Hui
作者单位	1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Yu, Jigang,Zhang, Xuecheng,et al. Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD[J]. PROTEIN AND PEPTIDE LETTERS,2018,25(4):362-367.
APA	Zhang, Wei.,Yu, Jigang.,Zhang, Xuecheng.,Peng, Hui.,Li, Xinxin.,...&Tu, Xiaoming.(2018).Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD.PROTEIN AND PEPTIDE LETTERS,25(4),362-367.
MLA	Zhang, Wei,et al."Ligand Induced Folding of the First Identified CBM69 Starch Binding Domain AmyP-SBD".PROTEIN AND PEPTIDE LETTERS 25.4(2018):362-367.