Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity

doi:10.1152/ajpcell.00292.2017

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity
	Xu, Xiaojuan 1,2; Guo, Mengmeng 3; Zhang, Na 1; Ye, Shoudong 3
刊名	AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
	2018-06-01
卷号	314 期号:6 页码:C712-C720
关键词	CHIR mouse embryonic stem cells self-renewal TCF3 TERRA
ISSN号	0363-6143
DOI	10.1152/ajpcell.00292.2017
通讯作者	Ye, Shoudong(shdye@126.com)
英文摘要	Although long noncoding RNAs (lncRNAs) are emerging as new modulators in the fate deeision of pi impotent stem cells, the functions of specific lncRNAs remain unclear. Here, we found that telomeric RNA (TERRA or TelRNA), one type of lncRNAs, is highly expressed in mouse embryonic stem cells (mESCs) but declines significantly upon differentiation. TERRA is induced by the Wnt/beta-catenin signaling pathway and can reproduce its self-renewal-promoting effect when overexpressed. Further studies revealed that T cell factor 3 (TCF3) is a potential downstream target of TERRA and mediates the effect of TERRA in mESC maintenance. TERRA inhibits TCF3 transcription, while enforced TCF3 expression abrogates the undifferentiated state of mESCs supported by TERRA. Accordingly, the transcripts of the pluripotency genes Esrrb, Tfcp2l1, and Klf2, repressed by TCF3 in mESCs, are increased in TERRA -overexpressing cells. Our study therefore highlights the important role of TERRA in mESC maintenance and also uncovers a mechanism by which TERRA promotes self-renewal. These data will expand our understanding of the pluripotent regulatory network of ESCs.
资助项目	Natural Science Foundation of China[31671535] ; Natural Science Foundation of China[B020702] ; Natural Science Foundation of Anhui Province[1508085SQC204] ; Scientific Research Startup Fund of Anhui University[J01006068]
WOS关键词	REPEAT-CONTAINING RNA ; PLURIPOTENCY NETWORK ; REGULATORY CIRCUITRY ; DIFFERENTIATION ; TERRA ; MICRORNAS ; MAINTAINS ; TRANSCRIPTION ; EXPRESSION ; REPRESSION
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	AMER PHYSIOLOGICAL SOC
WOS记录号	WOS:000441181200007
资助机构	Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; 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内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/37787]
专题	合肥物质科学研究院_中科院强磁场科学中心
通讯作者	Ye, Shoudong
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei, Anhui, Peoples R China 3.Anhui Univ, Sch Life Sci, Ctr Stem Cell & Translat Med, 111 Jiu Long Rd, Hefei 230601, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiaojuan,Guo, Mengmeng,Zhang, Na,et al. Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2018,314(6):C712-C720.
APA	Xu, Xiaojuan,Guo, Mengmeng,Zhang, Na,&Ye, Shoudong.(2018).Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,314(6),C712-C720.
MLA	Xu, Xiaojuan,et al."Telomeric noncoding RNA promotes mouse embryonic stem cell self-renewal through inhibition of TCF3 activity".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 314.6(2018):C712-C720.