Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system

doi:10.1016/j.gene.2019.02.037

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system
	Zhang, Wei 1; Liu, Shenghao 2; Li, Chengcheng 3; Zhang, Pengying 3; Zhang, Peiyu 1
刊名	GENE
	2019-05-15
卷号	696 页码:122-134
关键词	Antarctic moss Extremophile Flavonoids Salt stress Transcriptome
ISSN号	0378-1119
DOI	10.1016/j.gene.2019.02.037
英文摘要	Mosses are predominant terrestrial vegetation in Antarctica. Their distributions appear to be controlled more by water and salinity. The Antarctic moss Pohlia nutans can tolerate high salt stress. Here, high-throughput sequencing was employed to study the transcriptional characteristics of P. nutans under salt stress. Differentially expressed genes (DEGs) analysis showed that 1340 genes were significantly upregulated and 831 genes were markedly downregulated. The expression of representative DEGs including abscisic acid (ABA) and Jasmonates (JAs) pathway-related genes, antioxidant enzyme genes, and flavonoid biosynthesis-related genes were analyzed by real-time PCR and most were upregulated after salt stress. Furthermore, malondialdehyde (MDA) content was significantly increased after salt treatment. The levels of hydroxyl free radical (OH) first rose then quickly decreased. In addition, the activities of antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD), and the flavonoid content were enhanced after salt stress. Exogenous application of ABA, Methyl jasmonate (MeJA) or proanthocyanidins (PA) improved the performance of P. nutans in response to high salt stress. Furthermore, real-time PCR showed that ABA or MeJA treatment upregulated the gene expression of antioxidant and flavonoid biosynthesis-related enzymes. These results suggest that the responses of P. nutans under salt stress are involved in activating phytohormone signaling pathways which trigger two main antioxidant defense systems (i.e., antioxidant enzymes and flavonoids) for protecting cell and scavenging reactive oxygen species.
资助项目	Natural Science Foundation of Shandong Province[ZR2017MD013]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000463277600016
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/18354]
专题	业务部门_海洋生态研究中心
通讯作者	Liu, Shenghao
作者单位	1.Qingdao Univ, Sch Environm Sci & Engn, Qingdao 266071, Shangdong, Peoples R China 2.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China 3.Shandong Univ, Sch Life Sci, Natl Glycoengn Res Ctr, Qingdao 266237, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Liu, Shenghao,Li, Chengcheng,et al. Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system[J]. GENE,2019,696:122-134.
APA	Zhang, Wei,Liu, Shenghao,Li, Chengcheng,Zhang, Pengying,&Zhang, Peiyu.(2019).Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system.GENE,696,122-134.
MLA	Zhang, Wei,et al."Transcriptome sequencing of Antarctic moss under salt stress emphasizes the important roles of the ROS-scavenging system".GENE 696(2019):122-134.