Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)

doi:10.1016/j.jip.2010.05.016

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)
	Liu, Hongzhan 2,3; Zheng, Fengrong 1; Sun, Xiuqin ; Hong, Xuguang ; Dong, Shuanglin 2; Wang, Bo ; Tang, Xuexi 2; Wang, Yongqiang 4
刊名	JOURNAL OF INVERTEBRATE PATHOLOGY
	2010-11
卷号	105 期号:3 页码:236-242
关键词	Apostichopus japonicus Bacterial isolation and identification Virus Experimental infection
ISSN号	0022-2011
DOI	10.1016/j.jip.2010.05.016
英文摘要	The aquaculture of sea cucumbers Apostichopus japonicus (Selenka) has developed rapidly in China in recent years, but is increasingly affected by diseases such as skin ulceration and peristome tumescence. Previous studies on the pathogens causing these diseases focused largely on bacterial causes. In December 2008, we isolated four dominant bacterial species from lesions present in A. japonicus with the aforementioned diseases, from a farm in Yangkou (Qingdao, China). With two of these bacterial species, experimental infection of healthy A. japonicus resulted in the same disease symptoms that occurred in naturally infected A. japonicus. These two species were identified as Pseudoalteromonas sp. and Pseudoalteromonas tetraodonis. The early symptoms of infection for these bacterial species were ulcer spots on the dorsal skin and abdominal parapodia, followed by an increase in the number of ulcer spots or their merging into larger spots. Additionally, we isolated a spherical virus 100-250 nm in diameter and with a bilayer capsule, from A. japonicus with another disease from four different farms. By experimental infection with crude extracts of the virus, healthy laboratory-acclimatized A. japonicus developed the same symptoms as in natural infected cases. The early symptoms of viral infection comprised a decrease in tentacle activity, decay of dorsal papillate podia, peristome tumescence and abdominal ulceration. Our study demonstrates that the bacteria and virus were both responsible for skin ulceration and peristome tumescence in A. japonicus, but resulted in different early disease symptoms. (C) 2010 Published by Elsevier Inc.
资助项目	National Infrastructure of Natural Resources for Science and Technology Program of China[2004DKA30640]
WOS研究方向	Zoology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000283836000004
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4284]
专题	业务部门_海洋生态研究中心
作者单位	1.First Inst Oceanog SOA, Ecol Lab, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Coll Life Sci, Qingdao 266003, Peoples R China; 3.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China; 4.Shandong Prov Mari Culture Inst, Qingdao 266002, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hongzhan,Zheng, Fengrong,Sun, Xiuqin,et al. Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2010,105(3):236-242.
APA	Liu, Hongzhan.,Zheng, Fengrong.,Sun, Xiuqin.,Hong, Xuguang.,Dong, Shuanglin.,...&Wang, Yongqiang.(2010).Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka).JOURNAL OF INVERTEBRATE PATHOLOGY,105(3),236-242.
MLA	Liu, Hongzhan,et al."Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka)".JOURNAL OF INVERTEBRATE PATHOLOGY 105.3(2010):236-242.