Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress | |
Liu, Shenghao1,2; Ju, Jianfang1; Xia, Guangmin1 | |
刊名 | GENE |
2014-06-10 | |
卷号 | 543期号:1页码:145-152 |
关键词 | Abiotic stress Antarctic moss Real-time PCR Flavonoid hydroxylase Phylogenetic analysis |
ISSN号 | 0378-1119 |
DOI | 10.1016/j.gene.2014.03.026 |
英文摘要 | Flavonoids are ubiquitous plant secondary metabolites, and their hydroxylation pattern determines their color, stability, and antioxidant capacity. The hydroxylation pattern of the B-ring of flavonoids is determined by the activity of two members of cytochrome P450 protein (P450) family, the flavonoid 3 '-hydroxylase (F3 ' H) and flavonoid 3 ',5 '-hydroxylase (F3 ',5 ' H). However, they are still not well documented in lower plants such as bryophytes. We report the identification of gene encoding F3 ' H, F3',5 ' H from Antarctic moss Pohlia nutans and their transcriptional regulation under different stress conditions. Totally, sixteen cDNAs were isolated from P. nutans by RT-PCR and RACE techniques, all of which were predicted to code for F3 ' Hs or F3 ',5 ' Hs based on their annotations of Blast results. Amino acid alignment showed that they possessed the featured conserved domains of flavonoid hydroxylase, including proline-rich "hinge" region, EXXR motif, oxygen binding pocket motif, heme binding domain and substrate recognition sites. Phylogenetic analysis indicated that moss F3 ' Hs and F3 ',5 ' Hs were highly conserved and have independent evolution from the monocots, dicots and ferns. Meanwhile, real-time PCR analysis revealed that the expreSsion profiling of flavonoid hydroxylase genes was influenced by diverse abiotic stresses including cold, salinity, drought or UV-B radiation and plant hormone abscisic acid (ABA) or jasmonic acid apo treatment. Since 3 ',4 ',5 '-hydroxylated flavonoid-derivatives may serve a multitude of functions, including antioxidant activity and UV filters, the evolution and expression profile of flavonoid hydroxylase probably reflect the adaptive value of Antarctic moss in the acclimation of polar environment. (C)2014 Elsevier B.V. All rights reserved. |
资助项目 | National Transgenic Project[2013ZX08002002-003] |
WOS研究方向 | Genetics & Heredity |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000336355500020 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3821] |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Peoples R China; 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Substance, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Shenghao,Ju, Jianfang,Xia, Guangmin. Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress[J]. GENE,2014,543(1):145-152. |
APA | Liu, Shenghao,Ju, Jianfang,&Xia, Guangmin.(2014).Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress.GENE,543(1),145-152. |
MLA | Liu, Shenghao,et al."Identification of the flavonoid 3 '-hydroxylase and flavonoid 3 ',5 '-hydroxylase genes from Antarctic moss and their regulation during abiotic stress".GENE 543.1(2014):145-152. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论