Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

doi:10.1016/j.mrfmmm.2014.07.010

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	Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair
	Li, Chongjie 1; Ma, Li 2,3; Mou, Shanli 4; Wang, Yibin 1; Zheng, Zhou 1; Liu, Fangming 1; Qi, Xiaoqing 1; An, Meiling 1; Chen, Hao 1; Miao, Jinlai 1,5
刊名	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
	2015-03
卷号	773 页码:37-42
关键词	Chlamydomonas sp ICE-L UV Photolyase Photo-reactivation
ISSN号	0027-5107
DOI	10.1016/j.mrfmmm.2014.07.010
英文摘要	Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation. A putative photolyase was identified in the Chlamydomonas sp. ICE-L transcriptome. The complete cDNA sequence was obtained by RACE-PCR. This PHR encoding includes a polypeptide of 579 amino acids with clear photolyase signatures belonging to class II CPD-photolyases, sharing a high degree of homology with Chlamydomonas reinhardtii (68%). Real-time PCR was performed to investigate the potential DNA damage and responses following UVB exposure. CPD photolyase mRNA expression level increased over 50-fold in response to UVB radiation for 6 h. Using photolyase complementation assay, we demonstrated that DNA photolyase increased photo-repair more than 116-fold in Escherichia coil strain SY2 under 100 mu w/cm(2) UVB radiation. To determine whether photolyase is active in vitro, CPD photolyase was over-expressed. It was shown that pyrimidine dimers were split by the action of PHR2. This study reports the unique structure and high activity of the enzyme. These findings are relevant for further understanding of molecular mechanisms of photo-reactivation, and will accelerate the utilization of photolyase in the medical field. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	Open Research Fund of State Key Laboratory of Biological Fermentation Engineering of Beer[K2012007] ; Open Research Fund of State Key Laboratory of Biological Fermentation Engineering of Beer[K2012003]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000351979200006
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3692]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China; 3.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China; 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China; 5.State Key Lab Biol Fermentat Engn Beer Preparat, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Chongjie,Ma, Li,Mou, Shanli,et al. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2015,773:37-42.
APA	Li, Chongjie.,Ma, Li.,Mou, Shanli.,Wang, Yibin.,Zheng, Zhou.,...&Miao, Jinlai.(2015).Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,773,37-42.
MLA	Li, Chongjie,et al."Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 773(2015):37-42.