Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain

doi:10.1590/S1517-838246320131289

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
	Li, Jiang 1; Hu, Qiushi 2; Li, Yuquan 3; Xu, Yuan 3
刊名	BRAZILIAN JOURNAL OF MICROBIOLOGY
	2015-07-01
卷号	46 期号:3 页码:683-690
关键词	psychrophilic bacteria cold-adapted agarase purification characterization
ISSN号	1517-8382
DOI	10.1590/S1517-838246320131289
英文摘要	An extracellular beta-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 degrees C, respectively. However, it maintained as much as 85% of the maximum activities at 10 degrees C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
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资助项目	Basic Scientific Research Funds of FIO, SOA[GY0214T09]
WOS研究方向	Microbiology
语种	英语
出版者	SOC BRASILEIRA MICROBIOLOGIA
WOS记录号	WOS:000360074700007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3637]
专题	业务部门_海洋生态研究中心
作者单位	1.First Inst Oceanog, Key Lab Marine Bioact Subst, Qingdao, Peoples R China; 2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao, Peoples R China; 3.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Jiang,Hu, Qiushi,Li, Yuquan,et al. Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain[J]. BRAZILIAN JOURNAL OF MICROBIOLOGY,2015,46(3):683-690.
APA	Li, Jiang,Hu, Qiushi,Li, Yuquan,&Xu, Yuan.(2015).Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain.BRAZILIAN JOURNAL OF MICROBIOLOGY,46(3),683-690.
MLA	Li, Jiang,et al."Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain".BRAZILIAN JOURNAL OF MICROBIOLOGY 46.3(2015):683-690.