Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas)

doi:10.1016/j.fsi.2016.09.040

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	Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas)
	Cong, Bailin 1,2; Chai, Yingmei 3; Wang, Bo 2; Liu, Shenghao 2; Shen, Jihong 2; Wang, Nengfei 1,2; Zhang, Quanqi 1; Huang, Xiaohang 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017-01
卷号	60 页码:426-435
关键词	Voltage-gated potassium ion channels Sea perch Lateolabrax japonicas Macrophages LPS Immune function
ISSN号	1050-4648
DOI	10.1016/j.fsi.2016.09.040
英文摘要	Potassium ion channels are one of the most diversely and widely distributed channels, which are involved in all kinds of physiological functions in both excitable and non-excitable cells. The expression of voltage-gated potassium ion (Kv) channels is highly variable according to the state of macrophages activation. Macrophages have an important function in innate immunity against intruding pathogens. They produce a variety of inflammatory and immunoactive molecules that modulate imflammatory responses. Here we show that blockade of K+ channels by non-selective Kv channel inhibitor tetraethylammonium chloride (TEA), and 4-aminopyridine (4-AP) inhibited proinflammatory cytokines expression, cell proliferation, and reactive oxygen species (ROS) production in LPS-stimulated macrophages of Sea perch (Lateolabrax japonicas). Then we isolated four Kv channels genes (spKv1.1, spKv1.2, spKv1.5 and spKv3.1) in LPS-activated fish macrophages. These channels genes were up-regulated after LPS stimulation except spKv3.1, which remained unchanged during the test. The results of this study indicate that Kv channels could be required for modulating the immune function of fish macrophages. (C) 2016 Elsevier Ltd. All rights reserved.
资助项目	State 863 High-Technology R&D Project of China[2012AA10A408]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000392770000047
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3270]
专题	业务部门_海洋生态研究中心
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China; 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China; 3.Shandong Univ Weihai, Marine Coll, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Cong, Bailin,Chai, Yingmei,Wang, Bo,et al. Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas)[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:426-435.
APA	Cong, Bailin.,Chai, Yingmei.,Wang, Bo.,Liu, Shenghao.,Shen, Jihong.,...&Huang, Xiaohang.(2017).Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas).FISH & SHELLFISH IMMUNOLOGY,60,426-435.
MLA	Cong, Bailin,et al."Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas)".FISH & SHELLFISH IMMUNOLOGY 60(2017):426-435.