Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system | |
Wang, Hongying1; Chi, Zhe1; Cong, Ying1; Wang, Zhuangzhuang1; Jiang, Fei1; Geng, Jiayue1; Zhang, Peng2; Ju, Peng3; Dong, Quanjiang4,5; Liu, Chenguang1 | |
刊名 | RSC ADVANCES
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2018 | |
卷号 | 8期号:57页码:32454-32460 |
ISSN号 | 2046-2069 |
DOI | 10.1039/c8ra04819c |
英文摘要 | An aptamer-based fluorescence assay for culture-independent detection of Pseudomonas aeruginosa was developed. This assay was enabled by highly specific aptamers conjugated with photoluminescent carbon dots (CDs) as the fluorescent probe and graphene oxide (GO) as the quencher. Specially, high-throughput sequencing was achieved during systematic evolution of ligands via exponential enrichment (SELEX) for accurate recognition of aptamers. This assay displayed high specificity towards P. aeruginosa and was resistant to interference by other ubiquitous bacteria including Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Clostridium perfringens. After the conditions were optimized, this assay achieved a wide detection range for P. aeruginosa varying from 10(1) CFU mL(-1) to 10(7) CFU mL(-1). Notably, it approached an excellent detection limit as low as 9 CFU mL(-1). Therefore, this fluorescence assay was considered successfully developed for highly sensitive detection of P. aeruginosa. This assay also detected the contamination of P. aeruginosa in tap water and commercial bottled water, thereby suggesting its potential application in real water samples. |
资助项目 | National Natural Science Foundation of China[31500029] |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000448348200008 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3023] ![]() |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; 2.Chinese Acad Agr Sci, Res Ctr Plant Funct Components, Tobacco Res Inst, Qingdao 266001, Peoples R China; 3.SOA, Key Lab Marine Bioact Subst & Analyt Technol, Marine Ecol Ctr, Inst Oceanog 1, 6 Xianxialing Rd, Qingdao 266061, Peoples R China; 4.Qingdao Municipal Hosp, Cent Lab, Qingdao 266071, Peoples R China; 5.Qingdao Municipal Hosp, Dept Gastroenterol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Hongying,Chi, Zhe,Cong, Ying,et al. Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system[J]. RSC ADVANCES,2018,8(57):32454-32460. |
APA | Wang, Hongying.,Chi, Zhe.,Cong, Ying.,Wang, Zhuangzhuang.,Jiang, Fei.,...&Liu, Chenguang.(2018).Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system.RSC ADVANCES,8(57),32454-32460. |
MLA | Wang, Hongying,et al."Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system".RSC ADVANCES 8.57(2018):32454-32460. |
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