Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system
Wang, Hongying1; Chi, Zhe1; Cong, Ying1; Wang, Zhuangzhuang1; Jiang, Fei1; Geng, Jiayue1; Zhang, Peng2; Ju, Peng3; Dong, Quanjiang4,5; Liu, Chenguang1
刊名RSC ADVANCES
2018
卷号8期号:57页码:32454-32460
ISSN号2046-2069
DOI10.1039/c8ra04819c
英文摘要

An aptamer-based fluorescence assay for culture-independent detection of Pseudomonas aeruginosa was developed. This assay was enabled by highly specific aptamers conjugated with photoluminescent carbon dots (CDs) as the fluorescent probe and graphene oxide (GO) as the quencher. Specially, high-throughput sequencing was achieved during systematic evolution of ligands via exponential enrichment (SELEX) for accurate recognition of aptamers. This assay displayed high specificity towards P. aeruginosa and was resistant to interference by other ubiquitous bacteria including Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Clostridium perfringens. After the conditions were optimized, this assay achieved a wide detection range for P. aeruginosa varying from 10(1) CFU mL(-1) to 10(7) CFU mL(-1). Notably, it approached an excellent detection limit as low as 9 CFU mL(-1). Therefore, this fluorescence assay was considered successfully developed for highly sensitive detection of P. aeruginosa. This assay also detected the contamination of P. aeruginosa in tap water and commercial bottled water, thereby suggesting its potential application in real water samples.

资助项目National Natural Science Foundation of China[31500029]
WOS研究方向Chemistry
语种英语
出版者ROYAL SOC CHEMISTRY
WOS记录号WOS:000448348200008
内容类型期刊论文
源URL[http://ir.fio.com.cn/handle/2SI8HI0U/3023]  
专题业务部门_海洋生态研究中心
作者单位1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China;
2.Chinese Acad Agr Sci, Res Ctr Plant Funct Components, Tobacco Res Inst, Qingdao 266001, Peoples R China;
3.SOA, Key Lab Marine Bioact Subst & Analyt Technol, Marine Ecol Ctr, Inst Oceanog 1, 6 Xianxialing Rd, Qingdao 266061, Peoples R China;
4.Qingdao Municipal Hosp, Cent Lab, Qingdao 266071, Peoples R China;
5.Qingdao Municipal Hosp, Dept Gastroenterol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Wang, Hongying,Chi, Zhe,Cong, Ying,et al. Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system[J]. RSC ADVANCES,2018,8(57):32454-32460.
APA Wang, Hongying.,Chi, Zhe.,Cong, Ying.,Wang, Zhuangzhuang.,Jiang, Fei.,...&Liu, Chenguang.(2018).Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system.RSC ADVANCES,8(57),32454-32460.
MLA Wang, Hongying,et al."Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system".RSC ADVANCES 8.57(2018):32454-32460.
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