Molecular cloning and functional analysis of a Delta(12)-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L

doi:10.1007/s13205-019-1858-6

	Molecular cloning and functional analysis of a Delta(12)-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L
	He, Yingying 1; Zheng, Zhou 1,2,3; An, Meiling 1; Chen, Hao 1; Qu, Changfeng 2; Liu, Fangming 2; Wang, Yibin 2; Miao, Jinlai 1,2,3; Hou, Xuguang 4
刊名	3 BIOTECH
	2019-08-10
卷号	9 期号:9
关键词	Chlamydomonas sp ICE-L Fatty acid desaturase Polyunsaturated fatty acid Temperature stress Salt stress
ISSN号	2190-572X
DOI	10.1007/s13205-019-1858-6
英文摘要	Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Delta(12)-fatty acid desaturase (FAD)-encoding gene (Delta(12)CiFAD), 1269 bp in size, was cloned from Chlamydomonas sp. ICE-L. Bioinformatics analysis showed that Delta(12)CiFAD-encoded protein was homologous to known FADs with conserved histidine motifs, and localized to the chloroplast. Functional analysis of Delta(12)CiFAD indicated that recombinant Synechococcus 6803 expressing Delta(12)CiFAD could accumulate C18:2, whereas recombinant Saccharomyces cerevisiae expressing this enzyme could not accumulate C18:2 or any other new fatty acids. These results indicate that Delta(12)CiFAD is a functional enzyme in the chloroplast that can adjust Chlamydomonas sp. ICE-L cell membrane fluidity to adapt to Antarctic extreme low-temperature environments, which give us insights into the frigostable and cold-resistant mechanisms of hypothermic organisms.
资助项目	Youth Fund Project of Shandong Natural Science Foundation[ZR2017QD008]
WOS关键词	SACCHAROMYCES-CEREVISIAE ; FATTY-ACIDS ; EXPRESSION ; TEMPERATURE ; METABOLISM ; DELTA-12 ; GROWTH ; LIPIDS ; GENES ; GREEN
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000480577400002
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/30085]
专题	自然资源部第一海洋研究所
通讯作者	Miao, Jinlai; Hou, Xuguang
作者单位	1.Qingdao Univ, Sch Basic Med, Qingdao 266071, Shandong, Peoples R China 2.Minist Nat Resource, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Shandong, Peoples R China 3.Lab Marine Drugs & Bioprod Qingdao, Natl Lab Marine Sci & Technol, Qingdao 266237, Shandong, Peoples R China 4.Shandong Univ Weihai, Marine Coll, Weihai 264209, Shandong, Peoples R China
推荐引用方式 GB/T 7714	He, Yingying,Zheng, Zhou,An, Meiling,et al. Molecular cloning and functional analysis of a Delta(12)-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L[J]. 3 BIOTECH,2019,9(9).
APA	He, Yingying.,Zheng, Zhou.,An, Meiling.,Chen, Hao.,Qu, Changfeng.,...&Hou, Xuguang.(2019).Molecular cloning and functional analysis of a Delta(12)-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.3 BIOTECH,9(9).
MLA	He, Yingying,et al."Molecular cloning and functional analysis of a Delta(12)-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L".3 BIOTECH 9.9(2019).