Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

	Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3
	Yu Leiye 1; Sun Guojie 1; Wei Jingfang 1; Wang Yingze 1; Du Chao 1; Li Jiang 2
刊名	Chinese Journal of Oceanology and Limnology
	2016
卷号	34 期号:5 页码:1064-1071
关键词	exopolysaccharide (EPS) Antarctic bacterium RAW264.7 TLR4 NF-kappaB
ISSN号	0254-4059
英文摘要	An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF)alpha secretion, and phagocytic ability. Furthermore, TAK- 242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and IkappaBalpha degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-kappaB signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.
资助项目	Supported by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs ; the National Science & Technology Major Project ; the Hebei Province Science & Technology Support Program ; the National Natural Science Foundation of China
WOS研究方向	Science & Technology - Other Topics
语种	英语
CSCD记录号	CSCD:5773051
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/29938]
专题	自然资源部第一海洋研究所
作者单位	1.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China 2.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China 3.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China 4.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China 5.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China 6.First Institute of Oceanography, State Oceanic Administration (SOA), Key Laboratory of Marine Bioactive Substances,State Oceanic Administration (SOA), Qingdao, Shandong 266061, China
推荐引用方式 GB/T 7714	Yu Leiye,Sun Guojie,Wei Jingfang,et al. Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3[J]. Chinese Journal of Oceanology and Limnology,2016,34(5):1064-1071.
APA	Yu Leiye,Sun Guojie,Wei Jingfang,Wang Yingze,Du Chao,&Li Jiang.(2016).Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3.Chinese Journal of Oceanology and Limnology,34(5),1064-1071.
MLA	Yu Leiye,et al."Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3".Chinese Journal of Oceanology and Limnology 34.5(2016):1064-1071.