Cloud point extraction coupled with HPLC-DAD for the determination of genistein and daidzein in river water

doi:10.1039/c3ay40595h

	Cloud point extraction coupled with HPLC-DAD for the determination of genistein and daidzein in river water
	Wang, Chun 1; Wang, Ling 1; Zhao, Quansheng 1; Chen, Junhui 2; Zheng, Li 2; Zheng, Minggang 2; Zhang, Rutan 2; Wang, Zijian 1
刊名	ANALYTICAL METHODS
	2013
卷号	5 期号:15 页码:3688-3692
ISSN号	1759-9660
DOI	10.1039/c3ay40595h
英文摘要	Applying cloud-point extraction (CPE) as a preconcentration method for the determination of trace level pollutants has gained increased attention in recent years. This work aimed at developing an analytical method for the determination of genistein (GEN) and daidzein (DAI) in environmental water samples by the use of high-performance liquid chromatography separation and diode array detection (HPLC-DAD). We selected the non-ionic surfactant Triton X-114 as the extraction solvent. The parameters such as the concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time which affect extraction efficiency were evaluated and optimized. The developed method showed overall good performance with enrichment factors of 80, 144 for GEN and DAI, respectively, and detection limits of 3.77 ng mL(-1) for GEN and 2.23 ng mL(-1) for DAI in 30 mL water sample under the optimum conditions. The recoveries for GEN and DAI were all above 91% and RSD for GEN and DAI were 7.41% and 7.87%, respectively. Our study shows that the proposed method can be successfully adopted for the determination of trace amounts of GEN and DAI in river water.
资助项目	Natural Science Foundation of Shandong Province[ZR2010BQ025]
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; POLYCYCLIC AROMATIC-HYDROCARBONS ; MICELLE-MEDIATED EXTRACTION ; MEDAKA ORYZIAS-LATIPES ; CAPILLARY-ELECTROPHORESIS ; MEDICINAL PREPARATIONS ; PUERARIAE-RADIX ; ELECTROCHEMICAL DETECTION ; PHASE SEPARATIONS ; ORGANIC-COMPOUNDS
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000321625900013
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27491]
专题	自然资源部第一海洋研究所
通讯作者	Wang, Ling
作者单位	1.Qingdao Univ, Coll Chem & Chem Engn & Environm, Qingdao 266071, Peoples R China 2.First Inst Oceanog, State Ocean Adm, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chun,Wang, Ling,Zhao, Quansheng,et al. Cloud point extraction coupled with HPLC-DAD for the determination of genistein and daidzein in river water[J]. ANALYTICAL METHODS,2013,5(15):3688-3692.
APA	Wang, Chun.,Wang, Ling.,Zhao, Quansheng.,Chen, Junhui.,Zheng, Li.,...&Wang, Zijian.(2013).Cloud point extraction coupled with HPLC-DAD for the determination of genistein and daidzein in river water.ANALYTICAL METHODS,5(15),3688-3692.
MLA	Wang, Chun,et al."Cloud point extraction coupled with HPLC-DAD for the determination of genistein and daidzein in river water".ANALYTICAL METHODS 5.15(2013):3688-3692.