Evidence of Coexistence of C-3 and C-4 Photosynthetic Pathways in a Green-Tide-Forming Alga, Ulva prolifera

doi:10.1371/journal.pone.0037438

	Evidence of Coexistence of C-3 and C-4 Photosynthetic Pathways in a Green-Tide-Forming Alga, Ulva prolifera
	Xu, Jianfang 1; Fan, Xiao 2; Zhang, Xiaowen 2; Xu, Dong 2; Mou, Shanli 1; Cao, Shaona 3; Zheng, Zhou 1; Miao, Jinlai 1; Ye, Naihao 2
刊名	PLOS ONE
	2012-05-16
卷号	7 期号:5
ISSN号	1932-6203
DOI	10.1371/journal.pone.0037438
英文摘要	Ulva prolifera, a typical green-tide-forming alga, can accumulate a large biomass in a relatively short time period, suggesting that photosynthesis in this organism, particularly its carbon fixation pathway, must be very efficient. Green algae are known to generally perform C-3 photosynthesis, but recent metabolic labeling and genome sequencing data suggest that they may also perform C-4 photosynthesis, so C-4 photosynthesis might be more wide-spread than previously anticipated. Both C-3 and C-4 photosynthesis genes were found in U. prolifera by transcriptome sequencing. We also discovered the key enzymes of C-4 metabolism based on functional analysis, such as pyruvate orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPC), and phosphoenolpyruvate carboxykinase (PCK). To investigate whether the alga operates a C-4-like pathway, the expression of rbcL and PPDK and their enzyme activities were measured under various forms and intensities of stress (differing levels of salinity, light intensity, and temperature). The expression of rbcL and PPDK and their enzyme activities were higher under adverse circumstances. However, under conditions of desiccation, the expression of rbcL and ribulose-1, 5-biphosphate carboxylase (RuBPCase) activity was lower, whereas that of PPDK was higher. These results suggest that elevated PPDK activity may alter carbon metabolism and lead to a partial operation of C-4-type carbon metabolism in U. prolifera, probably contributing to its wide distribution and massive, repeated blooms in the Yellow Sea.
资助项目	National Marine Public Welfare Research Project[200805069]
WOS关键词	RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE ; MULTIPLE SEQUENCE ALIGNMENT ; CARBON ASSIMILATION ; PHAEODACTYLUM-TRICORNUTUM ; CO2/O2 SPECIFICITY ; MARINE DIATOMS ; KRANZ ANATOMY ; METABOLISM ; EVOLUTIONARY ; GENOME
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000305341300115
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26872]
专题	自然资源部第一海洋研究所
通讯作者	Xu, Jianfang
作者单位	1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Substance, Qingdao, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China 3.Qingdao Agr Univ, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jianfang,Fan, Xiao,Zhang, Xiaowen,et al. Evidence of Coexistence of C-3 and C-4 Photosynthetic Pathways in a Green-Tide-Forming Alga, Ulva prolifera[J]. PLOS ONE,2012,7(5).
APA	Xu, Jianfang.,Fan, Xiao.,Zhang, Xiaowen.,Xu, Dong.,Mou, Shanli.,...&Ye, Naihao.(2012).Evidence of Coexistence of C-3 and C-4 Photosynthetic Pathways in a Green-Tide-Forming Alga, Ulva prolifera.PLOS ONE,7(5).
MLA	Xu, Jianfang,et al."Evidence of Coexistence of C-3 and C-4 Photosynthetic Pathways in a Green-Tide-Forming Alga, Ulva prolifera".PLOS ONE 7.5(2012).