A Novel Receptor-like Kinase (PnRLK-1) from the Antarctic Moss Pohlia nutans Enhances Salt and Oxidative Stress Tolerance

doi:10.1007/s11105-014-0823-0

	A Novel Receptor-like Kinase (PnRLK-1) from the Antarctic Moss Pohlia nutans Enhances Salt and Oxidative Stress Tolerance
	Zhang, Pengying 1,2; Zhang, Zhaohui 3; Wang, Jing 1,2; Cong, Bailin 3; Chen, Kaoshan 1,2; Liu, Shenghao 1,2,3
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2015-08
卷号	33 期号:4 页码:1156-1170
关键词	Abiotic stress Bryophyte Protein kinase Phenotype Psychrophile
ISSN号	0735-9640
DOI	10.1007/s11105-014-0823-0
英文摘要	Plant receptor-like kinases (RLKs) are a group of conserved signaling components that regulate developmental programs and responses to biotic and abiotic stresses. However, only limited information is available on the functions of RLKs in bryophytes. Here, we described the isolation and functional characterization of a RLK gene (PnRLK-1) from the Antarctic moss Pohlia nutans. Sequence alignment and phylogenetic analysis showed that PnRLK-1 was homologous to the cytoplasmic-type RLKs. In addition, subcellular localization analysis in which the transient expression of PnRLK1-green fluorescent protein was observed in Arabidopsis thaliana mesophyll protoplasts revealed that PnRLK-1 was localized mainly in cytoplasm and a little on plasma membrane. Real-time PCR analysis showed that PnRLK-1 transcriptions were upregulated by mock cold, drought, high salinity, and exogenously supplied abscisic acid (ABA) or methyl jasmonate (MeJA). Overexpression of PnRLK-1 in transgenic Arabidopsis resulted in enhanced plant tolerance to high salinity and H2O2 stresses, and increased plant sensitivity to ABA. Furthermore, the expression levels of stress-responsive genes in transgenic Arabidopsis were also upregulated, including the genes of ABA-dependent signaling pathway (AtABI4, AtABI5, and AtMYB2) and reactive oxygen species (ROS)-scavenging enzymes (AtAPX1, AtZAT10, and AtCAT1). Therefore, we proposed that PnRLK-1 was a novel RLK involving in the adaptation of the Antarctic moss to the polar environment, increasing the salt and oxidative tolerance as well as the ABA sensitivity.
资助项目	Chinese Polar Environment Comprehensive Investigation & Assessment Programmes[CHINARE2013-01-06]
WOS关键词	ABSCISIC-ACID ; ABIOTIC STRESS ; GENE FAMILY ; PHYSCOMITRELLA-PATENS ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS ; HYDROGEN-PEROXIDE ; DROUGHT TOLERANCE ; PROTEIN-KINASES ; INNATE IMMUNITY
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000360392400034
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26827]
专题	自然资源部第一海洋研究所
通讯作者	Liu, Shenghao
作者单位	1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 2.Shandong Univ, Coll Life Sci, Jinan 250100, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Pengying,Zhang, Zhaohui,Wang, Jing,et al. A Novel Receptor-like Kinase (PnRLK-1) from the Antarctic Moss Pohlia nutans Enhances Salt and Oxidative Stress Tolerance[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(4):1156-1170.
APA	Zhang, Pengying,Zhang, Zhaohui,Wang, Jing,Cong, Bailin,Chen, Kaoshan,&Liu, Shenghao.(2015).A Novel Receptor-like Kinase (PnRLK-1) from the Antarctic Moss Pohlia nutans Enhances Salt and Oxidative Stress Tolerance.PLANT MOLECULAR BIOLOGY REPORTER,33(4),1156-1170.
MLA	Zhang, Pengying,et al."A Novel Receptor-like Kinase (PnRLK-1) from the Antarctic Moss Pohlia nutans Enhances Salt and Oxidative Stress Tolerance".PLANT MOLECULAR BIOLOGY REPORTER 33.4(2015):1156-1170.