Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment

doi:10.1099/ijsem.0.000722

	Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment
	Cui, Zhisong 1; Gao, Wei 1,2; Xu, Guangfei 1; Luan, Xiao 1; Li, Qian 1; Yin, Xiaofei 1; Huang, Deming 1; Zheng, Li 1,3
刊名	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
	2016-01
卷号	66 页码:353-359
ISSN号	1466-5026
DOI	10.1099/ijsem.0.000722
英文摘要	A rod-shaped, Gram-stain-negative, slightly halotolerant bacterium, designated strain D15-8P(T), was isolated from a sediment sample from the South China Sea. The strain could grow in NaCl concentrations ranging from 0.5 % to 10 % (w/v) (optimum 0.5-1.5 %), and could be cultivated at 10-40 degrees C (optimum 25 degrees C) and pH 5.5-9.5 (optimum pH 7.0-8.0). The strain was positive for catalase, oxidase, and hydrolysis of Tween 80, but negative for hydrolysis of DNA and gelatin, nitrite reduction, indole production, Voges-Proskauer reaction, and methyl red test. Strain D15-8P(T) could biodegrade naphthalene, phenanthrene, and anthracene. The major respiratory quinone was Q-9. The main cellular fatty acids were C-12 : 0 (11.5 %), C-14 : 0 3-methyl (22.0 %), C-16 : 0 (19.2 %), C-16 : 1 omega 9c (22.9 %), and C-18 : 1 omega 9c (6.7 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G + C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA genes showed that strain D15-8P(T) was most closely related to Marinobacter maritimus JCM 12521(T) (98.5 % 16S rRNA gene sequence similarity), Marinobacter antarcticus CGMCC 1.10835(T) (98.1 %), Marinobacter lipolyticus DSM 15157(T) (97.1 %), and Marinobacter guineae CECT 7243(T) (97.0 %). Results of the gyrB gene analysis and DNA-DNA hybridization were both less than the cut-off values (90 % for gyrB gene sequence similarity and 70 % for DNA-DNA hybridization). On the basis of this taxonomic study using a polyphasic approach, strain D15-8P(T) represents a novel species of the genus Marinobacter, for which the name Marinobacter aromaticivorans sp. nov. is proposed. The type strain is D15-8P(T) (=CGMCC 1.11015(T) =KCTC 23781(T)).
资助项目	National Natural Science Foundation of China[41406127] ; National Natural Science Foundation of China[41476103] ; National Natural Science Foundation of China[41306074]
WOS关键词	HALOPHILIC BACTERIUM ; MARINE BACTERIUM ; GEN-NOV ; HALOTOLERANT ; SYSTEMATICS ; WATER ; DNA
WOS研究方向	Microbiology
语种	英语
出版者	MICROBIOLOGY SOC
WOS记录号	WOS:000371452900050
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26721]
专题	自然资源部第一海洋研究所
通讯作者	Cui, Zhisong
作者单位	1.State Ocean Adm China, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao, Peoples R China 2.Ocean Univ China, Coll Marine Life, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Cui, Zhisong,Gao, Wei,Xu, Guangfei,et al. Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2016,66:353-359.
APA	Cui, Zhisong.,Gao, Wei.,Xu, Guangfei.,Luan, Xiao.,Li, Qian.,...&Zheng, Li.(2016).Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,66,353-359.
MLA	Cui, Zhisong,et al."Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66(2016):353-359.