Simultaneous determination nucleosides in marine organisms using ultrasound-assisted extraction followed by hydrophilic interaction liquid chromatography-electrospray ionization time-of-flight mass spectrometry

doi:10.1002/jssc.201100421

	Simultaneous determination nucleosides in marine organisms using ultrasound-assisted extraction followed by hydrophilic interaction liquid chromatography-electrospray ionization time-of-flight mass spectrometry
	Zhao, Hengqiang 1,2; Chen, Junhui 1; Shi, Qian 1; Li, Xin 1; Zhou, Wenhui 1; Zhang, Daolai 3; Zheng, Li 1; Cao, Wei 1; Wang, Xiaoru 1; Lee, Frank Sen-Chun 1
刊名	JOURNAL OF SEPARATION SCIENCE
	2011-10
卷号	34 期号:19 页码:2594-2601
关键词	ESI-TOF/MS Hydrophilic interaction chromatography Marine organisms Nucleosides
ISSN号	1615-9306
DOI	10.1002/jssc.201100421
英文摘要	A new method has been developed based on ultrasound-assisted extraction (UAE) followed by hydrophilic interaction chromatography (HILIC) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) for the simultaneous determination of 16 nucleosides and nucleobases in medicinal extracts of various marine organisms. The separation was achieved on a Venusil HILIC column (250 x 4.6 mm id, 5 mu m) and gradient elution using a solution of acetonitrile and buffer (0.20% formic acid and 20 mmol/L ammonium acetate) as the mobile phase. Identification of the 16 target nucleosides and nucleobases was based on the retention time, UV spectra, and mass measurements of the protonated molecules ([M+H](+)) and main fragment ions (ESI-TOF/MS). In addition, non-target compounds of 2'-deoxyinosine and four other amino acids were also tentatively identified by ESI-TOF/MS. The 16 target compounds were quantified by HILIC-ESI-TOF/MS under optimized mass conditions. All calibration curves showed good linearity (r(2)>0.9951). The recoveries were 84.72-124.10%, and the limits of detection of the 16 target compounds were 0.6-130.0 ng/mL. The developed method was applied to quantify the target compounds in 15 batches of various marine organisms. The method has potential applicability for the identification and determination of highly polar and low-concentration active compounds in marine organisms.
资助项目	Ministry of Science and Technology of China[2007AA092001-10]
WOS关键词	CULTURED CORDYCEPS ; CAPILLARY-ELECTROPHORESIS ; FINGERPRINT ANALYSIS ; PESTICIDE-RESIDUES ; PHOTODIODE-ARRAY ; ESI-MS/MS ; BASES ; ALKALOIDS ; IDENTIFICATION ; NUCLEOTIDES
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000296141200006
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26704]
专题	自然资源部第一海洋研究所
通讯作者	Chen, Junhui
作者单位	1.SOA, Inst Oceanog 1, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China 2.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan, Peoples R China 3.Qingdao Inst Marine Geol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Hengqiang,Chen, Junhui,Shi, Qian,et al. Simultaneous determination nucleosides in marine organisms using ultrasound-assisted extraction followed by hydrophilic interaction liquid chromatography-electrospray ionization time-of-flight mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(19):2594-2601.
APA	Zhao, Hengqiang.,Chen, Junhui.,Shi, Qian.,Li, Xin.,Zhou, Wenhui.,...&Lee, Frank Sen-Chun.(2011).Simultaneous determination nucleosides in marine organisms using ultrasound-assisted extraction followed by hydrophilic interaction liquid chromatography-electrospray ionization time-of-flight mass spectrometry.JOURNAL OF SEPARATION SCIENCE,34(19),2594-2601.
MLA	Zhao, Hengqiang,et al."Simultaneous determination nucleosides in marine organisms using ultrasound-assisted extraction followed by hydrophilic interaction liquid chromatography-electrospray ionization time-of-flight mass spectrometry".JOURNAL OF SEPARATION SCIENCE 34.19(2011):2594-2601.