Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp at different growth phases

doi:10.1016/j.gene.2013.04.005

	Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp at different growth phases
	Zheng, Minggang 1; Tian, Jinhu 2; Yang, Guanpin 3; Zheng, Li 1; Chen, Guogang 2; Chen, Jiluan 2; Wang, Bo 1
刊名	GENE
	2013-07-10
卷号	523 期号:2 页码:117-121
关键词	Nannochloropsis sp. Transcriptome Growth phase Lipid accumulation
ISSN号	0378-1119
DOI	10.1016/j.gene.2013.04.005
英文摘要	Nannochloropsis sp. is an oil-rich microalga. The lipid content of cells at stationary growth phase was much higher than that of cells at logarithm growth phase, but very little information focusing on the abundances of gene transcripts concerning lipid accumulation is available. In present study, the second generation Illumina sequencing was used to investigate the genes and pathways of Nannochloropsis sp. in order to identify those involved in lipid metabolism. In total, 29,203 unigenes were obtained from logarithm and stationary growth phases. The transcripts of some unigenes were differentially abundant at two growth phases. Among the unigenes obtained, about 195 unigenes were involved in lipid metabolism and 315 unigenes were putatively transcription factors. These genes are a very useful resource for the future studies of Nannochloropsis sp. (C) 2013 Elsevier B.V. All rights reserved.
资助项目	Marine Charity Project[2013418022-8]
WOS关键词	LIPID-ACCUMULATION ; MICROALGAE ; LENGTH ; BIOSYNTHESIS ; SALINITY ; BIOFUELS ; DROUGHT ; DIATOMS ; STRESS
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000320424400001
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26654]
专题	自然资源部第一海洋研究所
通讯作者	Wang, Bo
作者单位	1.State Ocean Adm China, Inst Oceanog 1, Res Ctr Marine Ecol, Qingdao 266061, Peoples R China 2.Shihezi Univ, Coll Food, Shihezi 832000, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Minggang,Tian, Jinhu,Yang, Guanpin,et al. Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp at different growth phases[J]. GENE,2013,523(2):117-121.
APA	Zheng, Minggang.,Tian, Jinhu.,Yang, Guanpin.,Zheng, Li.,Chen, Guogang.,...&Wang, Bo.(2013).Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp at different growth phases.GENE,523(2),117-121.
MLA	Zheng, Minggang,et al."Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp at different growth phases".GENE 523.2(2013):117-121.