Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin sculpin

doi:10.1016/j.fsi.2017.12.069

	Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin sculpin
	Chai, Yingmei 1; Cong, Bailin 2; Yu, Shanshan 1; Liu, Yingying 1; Man, Xin 1; Wang, Lujie 1; Zhu, Qian 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-03
卷号	74 页码:620-626
关键词	TfLECT2 Roughskin sculpin Peritoneal leukocytes Immune response V. anguillarum
ISSN号	1050-4648
DOI	10.1016/j.fsi.2017.12.069
英文摘要	Leukocyte cell-derived chemotaxin 2 (LECT2) is a multi-functional protein that is mainly synthesized by the liver. However, its role in roughskin scalping is less known. Here, we cloned a leukocyte cell-derived chemotaxin 2 (TfLECT2) genes in the liver of roughskin scalping, 7'rachidermus fasciatus, and studied its possible role involved in the immune response against Vibrio anguillarum (V. anguillarum) of peritoneal lecukocytes under in vivo conditions. The cDNA sequence of TfLECT2 is 566 bp in size. Its deduced amino acid (aa) sequence comprises 151 residues, of which the first 16 residues form a putative signal peptide and 101 residues compose a typical peptidase M23 domain in the C-terminal region. The domain structure is conserved in all LECT2 proteins, which suggests a close phylogenetic relationship between TfLECT2 and LECT2 in other fish species. Real-time quantitative PCR analysis revealed that TfLECT2 gene expression was dramatically increased in liver after V. anguillarum stimulation. Subsequently, TfLECT2 was prokaryotic expressed and purified to prepare anti-TfLECT2 antibody. After V. anguillartan challenge, leukocytes recruitment and LECT2 levels in peritoneal exudates were increased, and positively correlated with each other. Moreover, recombinant TfLECT2 administration significantly improved immune responses after infection, principally in stimulating the recruitment, phagocytosis and respiratory burst of leukocytes at the site of infection; however, anti-TfLECT2 treatment neutralized these abilities. Therefore, TfLECT2 may trigger the early immune events of peritoneal leukocytes and it will be useful to induce innate immune response of fish.
资助项目	Ocean Park Conservation Foundation, Hong Kong (OPCFHK)[MM03_1617]
WOS关键词	CELL-DERIVED CHEMOTAXIN-2 ; AYU PLECOGLOSSUS-ALTIVELIS ; NEWLY RECOGNIZED PROTEIN ; 3 DISULFIDE BONDS ; MOLECULAR-CLONING ; TELEOST FISH ; EXPRESSION ANALYSIS ; HEPATOCELLULAR-CARCINOMA ; INFLAMMATORY RESPONSE ; DICENTRARCHUS-LABRAX
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000427344200066
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26432]
专题	自然资源部第一海洋研究所
通讯作者	Chai, Yingmei; Zhu, Qian
作者单位	1.Shandong Univ Weihai, Marine Coll, Weihai 264209, Peoples R China 2.State Ocean Adm, Marine Ecol Ctr, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Chai, Yingmei,Cong, Bailin,Yu, Shanshan,et al. Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin sculpin[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:620-626.
APA	Chai, Yingmei.,Cong, Bailin.,Yu, Shanshan.,Liu, Yingying.,Man, Xin.,...&Zhu, Qian.(2018).Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin sculpin.FISH & SHELLFISH IMMUNOLOGY,74,620-626.
MLA	Chai, Yingmei,et al."Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin sculpin".FISH & SHELLFISH IMMUNOLOGY 74(2018):620-626.