Analysis of Delta pH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp ICE-L

doi:10.1007/s00792-013-0532-x

	Analysis of Delta pH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp ICE-L
	Mou, Shanli 1; Zhang, Xiaowen 1; Ye, Naihao 1; Miao, Jinlai 2; Cao, Shaona 3; Xu, Dong 1; Fan, Xiao 1; An, Meiling 2
刊名	EXTREMOPHILES
	2013-05
卷号	17 期号:3 页码:477-484
关键词	Delta pH Xanthophyll cycle NPQ Antarctic sea ice alga Chlamydomonas sp ICE-L
ISSN号	1431-0651
DOI	10.1007/s00792-013-0532-x
英文摘要	Non-photochemical fluorescence quenching (NPQ) is mainly associated with the transthylakoid proton gradient (Delta pH) and xanthophyll cycle. However, the exact mechanism of NPQ is different in different oxygenic photosynthetic organisms. In this study, several inhibitors were used to study NPQ kinetics in the sea ice alga Chlamydomonas sp. ICE-L and to determine the functions of Delta pH and the xanthophyll cycle in the NPQ process. NH4Cl and nigericin, uncouplers of Delta pH, inhibited NPQ completely and zeaxanthin (Z) was not detected in 1 mM NH4Cl-treated samples. Moreover, Z and NPQ were increased in the samples containing N,N'-dicyclohexyl-carbodiimide (DCCD) under low light conditions. We conclude that Delta pH plays a major role in NPQ, and activation of the xanthophyll cycle is related to Delta pH. In dithiothreitol (DTT)-treated samples, no Z was observed and NPQ decreased. NPQ was completely inhibited when NH4Cl was added suggesting that part of the NPQ process is related to the xanthophyll cycle and the remainder depends on Delta pH. Moreover, lutein and beta-carotene were also essential for NPQ. These results indicate that NPQ in the sea ice alga Chlamydomonas sp. ICE-L is mainly dependent on Delta pH which affects the protonation of PSII proteins and de-epoxidation of the xanthophyll cycle, and the transthylakoid proton gradient alone can induce NPQ.
资助项目	National Marine Public Welfare Research Project[200805069]
WOS关键词	PHOTOPROTECTIVE ENERGY-DISSIPATION ; PHOTOSYSTEM-II ; GRANA MEMBRANES ; PSBS PROTEIN ; EXCESS LIGHT ; FLUORESCENCE ; PHOTOSYNTHESIS ; ZEAXANTHIN ; ANTENNA ; CHLOROPLASTS
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
出版者	SPRINGER JAPAN KK
WOS记录号	WOS:000318169900012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25956]
专题	自然资源部第一海洋研究所
通讯作者	Ye, Naihao
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 3.Qingdao Agr Univ, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Mou, Shanli,Zhang, Xiaowen,Ye, Naihao,et al. Analysis of Delta pH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp ICE-L[J]. EXTREMOPHILES,2013,17(3):477-484.
APA	Mou, Shanli.,Zhang, Xiaowen.,Ye, Naihao.,Miao, Jinlai.,Cao, Shaona.,...&An, Meiling.(2013).Analysis of Delta pH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp ICE-L.EXTREMOPHILES,17(3),477-484.
MLA	Mou, Shanli,et al."Analysis of Delta pH and the xanthophyll cycle in NPQ of the Antarctic sea ice alga Chlamydomonas sp ICE-L".EXTREMOPHILES 17.3(2013):477-484.