Uncovering a Glycosyltransferase Provides Insights into the Glycosylation Step during Macrolactin and Bacillaene Biosynthesis

doi:10.1002/cbic.201402384

	Uncovering a Glycosyltransferase Provides Insights into the Glycosylation Step during Macrolactin and Bacillaene Biosynthesis
	Qin, Wen 1; Liu, Yang 1; Ren, Pengfei 1; Zhang, Jun 1; Li, Huayue 1; Tian, Li 2,3; Li, Wenli 1
刊名	CHEMBIOCHEM
	2014-12-15
卷号	15 期号:18 页码:2747-2753
关键词	bacillaenes (BAEs) Bacillus marinus biosynthesis glycosylation glycosyltransferases macrolactins
ISSN号	1439-4227
DOI	10.1002/cbic.201402384
英文摘要	Macrolactins (MLNs) have unique structural patterns containing a 24-membered ring lactone and diverse bioactivities. The MLN skeleton is biosynthesized via a trans-acyl transferase (AT) type I polyketide synthase (PKS) pathway, but the tailoring steps are still unknown. Herein, we report the identification of a glycosyltransferase (GT) gene bmmGT1, which is located at different locus from the MLN gene cluster in the genome of marine-derived Bacillus marinus B-9987, and its functional characterization as an MLN GT, thus affording five novel MLNs analogues. Surprisingly, this GT is also capable of catalyzing the glycosylation of bacillaenes (BAEs), which are the prototypes of trans-AT polyketides, thus suggesting broad substrate flexibility. These results provide the first significant insights into the glycosylation step in MLN and BAE biosynthetic pathways.
资助项目	State Key Laboratory of Microbial Resources Program, Institute of Microbiology, CAS[SKLMR-20110601]
WOS关键词	VANCOMYCIN-RESISTANT ENTEROCOCCI ; AMYLOLIQUEFACIENS FZB42 ; STAPHYLOCOCCUS-AUREUS ; PEPTIDE DEFORMYLASE ; POLYKETIDE SYNTHASE ; ENZYMATIC-SYNTHESIS ; CATALYZED REACTIONS ; MARINE BACTERIUM ; PRODUCT ; GLUCOSIDES
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000346023500016
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25831]
专题	自然资源部第一海洋研究所
通讯作者	Li, Wenli
作者单位	1.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao 266003, SD, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, SD, Peoples R China 3.Qingdao Univ Sci & Technol, Qingdao 266042, SD, Peoples R China
推荐引用方式 GB/T 7714	Qin, Wen,Liu, Yang,Ren, Pengfei,et al. Uncovering a Glycosyltransferase Provides Insights into the Glycosylation Step during Macrolactin and Bacillaene Biosynthesis[J]. CHEMBIOCHEM,2014,15(18):2747-2753.
APA	Qin, Wen.,Liu, Yang.,Ren, Pengfei.,Zhang, Jun.,Li, Huayue.,...&Li, Wenli.(2014).Uncovering a Glycosyltransferase Provides Insights into the Glycosylation Step during Macrolactin and Bacillaene Biosynthesis.CHEMBIOCHEM,15(18),2747-2753.
MLA	Qin, Wen,et al."Uncovering a Glycosyltransferase Provides Insights into the Glycosylation Step during Macrolactin and Bacillaene Biosynthesis".CHEMBIOCHEM 15.18(2014):2747-2753.