Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis

doi:10.1016/j.talanta.2005.09.041

	Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis
	Chen, JH ; Wang, FM ; Lee, FSC ; Wang, XR ; Xie, MY
刊名	TALANTA
	2006-03-15
卷号	69 期号:1 页码:172-179
关键词	Salvia miltiorrhiza Bunge Danshen salvianolic acids salvianolic acid B HSCCC
ISSN号	0039-9140
DOI	10.1016/j.talanta.2005.09.041
英文摘要	High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale has been applied to separate and purify salvianolic acids from the water extract of Danshen, Salvia miltiorrhiza Bunge. High efficiency HSCCC separation was achieved on a two-phase solvent system composed of a mixture of n-hexane-ethyl acetate-water-methanol (1.5:5:5:1.5, v/v) by eluting the lower mobile phase at a flow-rate of 1.7 ml/min and a revolution of 850 rpm. A total of five well separated peaks were obtained in the HSCCC chromatogram, and their purities determined by HPLC-UV absorption. These peaks were characterized by, UV-vis spectra and ESI-MS, and the data compared with the reference standards. Salvianolic acid B was positively identified as one of the major peaks. Three of the remaining four peaks were also tentatively identified as rosmarinic acid, lithospermic acid, and salvianolic acid E, an isomer of salvianolic acid B, all are members of the salvianolic acids group. In a typical run, tens of milligrams of samples can be separated with high efficiency to yield tens of milligrams of purified materials with over 98% purity. HSCCC thus provides a cost-effective alternative to preparative scale HPLC for the semi-preparative scale separation and purification of salvianolic acids in Danshen. With appropriate modifications, the technique should also be applicable to other herbs in general. (c) 2005 Elsevier B.V. All rights reserved.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; PURIFICATION ; TANSHINONES ; FINGERPRINT
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000235510000027
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25742]
专题	自然资源部第一海洋研究所
通讯作者	Wang, XR
作者单位	1.Xiamen Univ, Minist Educ, Key Lab Analyt Sci, Fujian 361005, Peoples R China 2.Nanchang Univ, Minst Educ, Key Lab Food Sci, Jiangxi 330047, Peoples R China 3.SAO, Inst Oceanog 1, QingDao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Chen, JH,Wang, FM,Lee, FSC,et al. Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis[J]. TALANTA,2006,69(1):172-179.
APA	Chen, JH,Wang, FM,Lee, FSC,Wang, XR,&Xie, MY.(2006).Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis.TALANTA,69(1),172-179.
MLA	Chen, JH,et al."Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis".TALANTA 69.1(2006):172-179.