Biodegradation of hydrolyzed polyacrylamide by a Bacillus megaterium strain SZK-5: Functional enzymes and antioxidant defense mechanism

doi:10.1016/j.chemosphere.2019.05.143

	Biodegradation of hydrolyzed polyacrylamide by a Bacillus megaterium strain SZK-5: Functional enzymes and antioxidant defense mechanism
	Song, Tianwen 1,2; Li, Shanshan 1,2; Lu, Yifeng 3; Yan, Dong 1,2; Sun, Peiyan 4; Bao, Mutai 1,2; Li, Yang 1,5
刊名	CHEMOSPHERE
	2019-09
卷号	231 页码:184-193
关键词	Hydrolyzed polyacrylamide Biodegradation Bacillus megaterium Functional enzymes Crude oil Oxidative stress
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2019.05.143
英文摘要	Hydrolyzed polyacrylamide (HPAM) is the most widely used water-soluble linear polymer with high molecular weight in polymer flooding. Microbiological degradation is an environment-friendly and effective method of treating HPAM-containing oilfield produced water. In this study, a strain SZK-5 that could degrade HPAM was isolated from soil contaminated by oilfield produced water. Based on morphological, biochemical characteristics and 16S rDNA sequence homology analysis, the strain was identified as Bacillus megaterium. The biodegradation capability of strain SZK-5 was determined by incubation in a mineral salt medium (MSM) containing HPAM under different environmental conditions, showing 55.93% of the HPAM removed after 7 d of incubation under the optimum conditions ((NH4)(2)SO4 = 1667.9 mg L-1, temperature = 24.05 degrees C and pH = 8.19). Cytochrome P450 (CYP) and urease (URE) played significant roles in biological carbon and nitrogen removal, respectively. The strain SZK-5 could resist the damages caused by oxidative stress given by crude oil and HPAM. To our knowledge, this is the first report about the biodegradation of HPAM by B. megaterium. These results suggest that strain SZK-5 might be a new auxiliary microbiological resource for the biodegradation of HPAM residue in wastewater and soil. (C) 2019 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[51174181]
WOS关键词	ORGANIC POLLUTANTS ; WASTE-WATER ; SLUDGE ; BIOAVAILABILITY ; TOXICITY ; CAPACITY ; UREASE ; SOIL ; TRANSFORMATION ; BACTERIA
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000472694200022
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24546]
专题	自然资源部第一海洋研究所
通讯作者	Bao, Mutai
作者单位	1.Ocean Univ China, Inst Adv Ocean Study, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Shandong, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Shandong, Peoples R China 3.Dalhousie Univ, Dept Environm Sci, Halifax, NS, Canada 4.State Ocean Adm, North China Sea Environm Monitoring Ctr, Key Lab Marine Spill Oil Identificat & Damage Ass, Qingdao 266033, Shandong, Peoples R China 5.China Petrochem Corp Sinopec Grp, Beijing 100728, Peoples R China
推荐引用方式 GB/T 7714	Song, Tianwen,Li, Shanshan,Lu, Yifeng,et al. Biodegradation of hydrolyzed polyacrylamide by a Bacillus megaterium strain SZK-5: Functional enzymes and antioxidant defense mechanism[J]. CHEMOSPHERE,2019,231:184-193.
APA	Song, Tianwen.,Li, Shanshan.,Lu, Yifeng.,Yan, Dong.,Sun, Peiyan.,...&Li, Yang.(2019).Biodegradation of hydrolyzed polyacrylamide by a Bacillus megaterium strain SZK-5: Functional enzymes and antioxidant defense mechanism.CHEMOSPHERE,231,184-193.
MLA	Song, Tianwen,et al."Biodegradation of hydrolyzed polyacrylamide by a Bacillus megaterium strain SZK-5: Functional enzymes and antioxidant defense mechanism".CHEMOSPHERE 231(2019):184-193.