Construction of long-chain alkane degrading bacteria and its application in bioremediation of crude oil pollution

doi:10.1016/j.ijbiomac.2018.07.137

	Construction of long-chain alkane degrading bacteria and its application in bioremediation of crude oil pollution
	Meng, Long 1,2; Bao, Mutai 1,2; Sun, Peiyan 3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-11
卷号	119 页码:524-532
关键词	Crude oil biodegradation DNA and enzyme activity Co-biodegradation
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.07.137
英文摘要	In this study, a cloned almA gene was inserted into two plasmids PUC57 and pet-28a (+), and then transformed into Escherichia coli competent strains. The presence of almA was confirmed by PCR combined with restriction enzyme digestion. The results indicated two strains of PUC57/almA-E. coli and pet-28a (+)/almA-E. coli were all functioned on biodegradation long-chain alkanes, however, pet-28a (+)/almA-E. coli strain performed better and the speed of biodegradation was enhanced to about 12 mg L-1 h(-1) when compared with PUC57/almA-E. coli strain. Furthermore, degradation degree of n-C32 was much higher than other long-chain alkanes for two engineered E. coli strains. Our results further suggested that exoenzyme was only detected in pet-28a (+)/almA-E. coli and followed a decreasing order of extracellular enzyme > intracellular enzyme > periplasmic enzyme. Besides, co-biodegradation of petroleum hydrocarbons by Pseudomonas sp. B-1, Bacillus subtilis PH-1 with exoenzyme or pet-28a (+)/almA-E. coli posed enormous potential that could reach 70%-80% in oil pollution bioremediation. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	Program for Innovative Research Team in University[IRT1289]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000447682100059
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/10822]
专题	自然资源部第一海洋研究所
通讯作者	Bao, Mutai
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 3.State Ocean Adm, North China Sea Environm Monitoring Ctr, Key Lab Marine Spill Oil Identificat & Damage Ass, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Meng, Long,Bao, Mutai,Sun, Peiyan. Construction of long-chain alkane degrading bacteria and its application in bioremediation of crude oil pollution[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,119:524-532.
APA	Meng, Long,Bao, Mutai,&Sun, Peiyan.(2018).Construction of long-chain alkane degrading bacteria and its application in bioremediation of crude oil pollution.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,119,524-532.
MLA	Meng, Long,et al."Construction of long-chain alkane degrading bacteria and its application in bioremediation of crude oil pollution".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 119(2018):524-532.