Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy

	Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy
	Sun, Xiuqin ; Zheng, Fengrong ; Li, Jiye ; Wu, Xing'an ; Qu, Lingyun ; Zhang, Jinxing ; Hong, Xuguang
刊名	Gaojishu Tongxin/Chinese High Technology Letters
	2006-07-01
卷号	16 期号:7 页码:740-745
ISSN号	10020470
英文摘要	Total RNA was extracted from a gill-derived cell strain FG-9307 of the Japanese flounder, and a fragment of major capsid protein encoding gene (approximately 0.6 kb in length) was amplified using RT-PCR and cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV0.6 kb. This plasmid successfully achieved immediate expression after being transferred by liposome into the eukaryotic cell strain FEC. pEGFP-N2-LCDV0.6 kb was injected into Paralichthys olivaceus intramuscularly and T-lymphopoiesis, respiratory burst in peripheral blood, hind-intestines, spleen, head-kidney and antibodies against LCDV were evaluated. The results indicated that the plasmid of pEGFP-N2-LCDV0.6 kb could induce a unique humoral and cell-mediated immune response and possess the function of immune protection. Therefore, further studies are necessary for the development and application of a DNA vaccine.
语种	中文
出版者	Inst. of Scientific and Technical Information of China, Beijing, China
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/6848]
专题	自然资源部第一海洋研究所
作者单位	1.First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China; 2.Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China
推荐引用方式 GB/T 7714	Sun, Xiuqin,Zheng, Fengrong,Li, Jiye,et al. Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy[J]. Gaojishu Tongxin/Chinese High Technology Letters,2006,16(7):740-745.
APA	Sun, Xiuqin.,Zheng, Fengrong.,Li, Jiye.,Wu, Xing'an.,Qu, Lingyun.,...&Hong, Xuguang.(2006).Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy.Gaojishu Tongxin/Chinese High Technology Letters,16(7),740-745.
MLA	Sun, Xiuqin,et al."Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy".Gaojishu Tongxin/Chinese High Technology Letters 16.7(2006):740-745.