Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica

doi:10.1007/s13131-012-0183-2

	Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica
	Kan Guangfeng 1; Shi Cuijuan 1; Wang Xiaofei 1; Xie Qinju 1; Wang Min 1; Wang Xinlei 1; Mia Jinlai 2
刊名	ACTA OCEANOLOGICA SINICA
	2012-01
卷号	31 期号:1 页码:116-124
关键词	Antarctic ice microalga SOD enzymes ultra structure membrane fatty acids difference protein hypersaline acclimation
ISSN号	0253-505X
DOI	10.1007/s13131-012-0183-2
英文摘要	Antarctic ice microalga can survive and thrive in channels or pores containing high salinity in Antarctic ice layer. in this study, it was found that cell membrane permeability of green microalga Chlamydomonas sp. L4 from Antarctic sea ice was high in cells treated with hypersalinity due to the induction of active oxygen and radicals. However, increased super oxide dismutase (SOD) scavenged harmful free radicals effectively to keep cell membrane integrity. Also, the analysis of membrane fatty acids demonstrated the content of saturated fatty acids and monounsaturated fatty acids increased and polyunsaturated fatty acids decreased under the high-salt treatment for 14 d, which effectively reduced the membrane fluidity and minimized the injury to cell membrane. The morphological changes showed that hypersalinity induced the increase of cell volume and the consumption of starch granules. However, because of the increase in detoxification of vacuoles, electron-dense deposits and SOD activity under high-salt stress, the complete noninterference thylakoids, mitochondria and cell nucleus maintained cellular fundamental metabolism. Global-expression profiling of proteins showed eight protein spots disappeared, 18 protein spots decreased and 18 protein spots were enhanced after the high-salt shock obviously (P <0.05). One new peptide (pI 6.90; MW 51 kDa) was primarily confirmed as the processor of light reaction center protein CP43 in photosystem II, which increased photosynthesis ability of Chlamydomonas sp. L4 treated with high salinity.
资助项目	Fundamental Research Funds for the Central Universities[HIT.IBRSEM.2009148]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000299492700014
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4163]
专题	自然资源部第一海洋研究所
作者单位	1.Harbin Inst Technol, Sch Marine Sci & Technol, Weihai 264209, Peoples R China; 2.State Ocean Adm, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Kan Guangfeng,Shi Cuijuan,Wang Xiaofei,et al. Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica[J]. ACTA OCEANOLOGICA SINICA,2012,31(1):116-124.
APA	Kan Guangfeng.,Shi Cuijuan.,Wang Xiaofei.,Xie Qinju.,Wang Min.,...&Mia Jinlai.(2012).Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica.ACTA OCEANOLOGICA SINICA,31(1),116-124.
MLA	Kan Guangfeng,et al."Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica".ACTA OCEANOLOGICA SINICA 31.1(2012):116-124.