Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis

doi:10.1016/j.fsi.2012.04.011

	Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis
	Wang, Yanan 1; Liu, Xudong 1,2; Ma, Liman 1; Yu, Yan 3; Yu, Haiyang 1; Mohammed, Shafi 1; Chu, Guannan 1; Mu, Linlin 1; Zhang, Quanqi 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-08
卷号	33 期号:2 页码:213-219
关键词	Hepcidin Antimicrobial peptide Cynoglossus semilaevis Vibrio anguillarum Innate immunity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2012.04.011
英文摘要	Hepcidin, an antimicrobial peptide, has a dual function including innate immunity and iron regulation. Here, based on the sequence of an EST database, we have isolated and characterized a hepcidin gene (referred to as CsHepcidin) from half-smooth tongue sole (Cynoglossus semilaevis). Analysis of the coding regions indicated CsHepcidin gene comprised 3 exons and 2 introns. The putative CsHepcidin showed a great similarity to other hepcidin orthologues, particularly with respect to its 24 aa signal peptide, typical RX(K/R)R motif and eight conserved cysteine residues in the mature cationic peptide. Phylogenic analysis indicated that CsHepcidin was a hepcidin 1-type peptide of acanthopterygians, with highly homologous with Solea senegalensis hepcidin. In C semilaevis ontogeny, CsHepcidin mRNA was detected at a low level in unfertilized eggs, increased on 6 d after hatching, and decreased remarkably at meta-morphic stage. CsHepcidin transcripts showed a constitutive basal expression in most of the tissues, especially in liver. Challenge with formalin-inactivated Vibrio anguillarum led to significantly up-regulations of CsHepcidin gene in liver, head kidney and spleen in time-dependent manners. Biological activity analysis showed that recombinant CsHEP exhibited direct antimicrobial activity against bacterial pathogens in vitro, particularly showed strong activity against the principal fish pathogens, V. anguillarum and Edwardsiella tarda. All these results suggest that CsHepcidin may be involved in the initial response to invasion of microbial pathogens. Further exploration to elucidate the role of CsHepcidin in iron regulation and embryogenesis in C semilaevis are needed. (C) 2012 Elsevier Ltd. All rights reserved.
资助项目	National High-Tech Research and Development Program[2012AA100802]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000306632800007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4086]
专题	自然资源部第一海洋研究所
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China; 2.SOA, N China Sea Environm Monitoring Ctr, Qingdao 266033, Peoples R China; 3.Huainan Normal Univ, Dept Life Sci, Huainan 232001, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanan,Liu, Xudong,Ma, Liman,et al. Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):213-219.
APA	Wang, Yanan.,Liu, Xudong.,Ma, Liman.,Yu, Yan.,Yu, Haiyang.,...&Zhang, Quanqi.(2012).Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis.FISH & SHELLFISH IMMUNOLOGY,33(2),213-219.
MLA	Wang, Yanan,et al."Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):213-219.