HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China

doi:10.1007/s11802-014-2448-1

	HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China
	Liu Shuxia 1,2; Yao Peng 1,3,4; Yu Zhigang 1,4; Li Dong 1,5; Deng Chunmei 6; Zhen Yu 7
刊名	JOURNAL OF OCEAN UNIVERSITY OF CHINA
	2014-12
卷号	13 期号:6 页码:941-950
关键词	harmful algal bloom species high performance liquid chromatography chemotaxonomy cellular pigment density pigment ratios
ISSN号	1672-5182
DOI	10.1007/s11802-014-2448-1
英文摘要	Chemotaxonomy based on diagnostic pigments is now a routine tool for macroscopic determination of the composition and abundance of phytoplankton in various aquatic environments. Since the taxonomic capability of this method depends on the relationships between diagnostic pigments and chlorophyll a of classified groups, it is critical to calibrate it by using pigment relationships obtained from representative and/or dominant species local to targeted investigation area. In this study, pigment profiles of 31 harmful algal bloom (HAB) species isolated from the coastal sea areas of China were analyzed with high performance liquid chromatography (HPLC). Pigment compositions, cellular pigment densities and ratios of pigments to chlorophyll a were determined and calculated. Among all these species, 25 kinds of pigments were detected, of which fucoxanthin, peridinin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, and antheraxanthin were diagnostic pigments. Cellular pigment density was basically independent of species and environmental conditions, and therefore was recommended as a bridge to compare the results of HPLC-CHEMTAX technique with the traditional microscopy method. Pigment ratios of algal species isolated from the coast of China, especially the diagnostic pigment ratios, were higher than those from other locations. According to these results, pigment ratio ranges of four classes of phytoplankton common off the coast of China were summarized for using in the current chemotaxonomic method. Moreover, the differences of pigments ratios among different species under the same culturing conditions were consistent with their biological differences. Such differences have the potential to be used to classify the phytoplankton below class, which is meaningful for monitoring HABs by HPLC-CHEMTAX.
资助项目	National Marine Public Welfare Research Project of China[201205031]
WOS研究方向	Oceanography
语种	英语
出版者	OCEAN UNIV CHINA
WOS记录号	WOS:000344788700008
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3752]
专题	自然资源部第一海洋研究所
作者单位	1.Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China; 2.Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China; 3.Qingdao Collaborat Innovat Ctr Marine Sci & Techn, Qingdao 266100, Peoples R China; 4.Ocean Univ China, Inst Marine Organ Geochem, Qingdao 266100, Peoples R China; 5.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China; 6.State Ocean Adm, Key Lab Marine Spill Oil Identificat & Damage Ass, Qingdao 266033, Peoples R China; 7.Minist Educ, Key Lab Marine Environm & Ecol, Qingdao 266100, Peoples R China
推荐引用方式 GB/T 7714	Liu Shuxia,Yao Peng,Yu Zhigang,et al. HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(6):941-950.
APA	Liu Shuxia,Yao Peng,Yu Zhigang,Li Dong,Deng Chunmei,&Zhen Yu.(2014).HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China.JOURNAL OF OCEAN UNIVERSITY OF CHINA,13(6),941-950.
MLA	Liu Shuxia,et al."HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China".JOURNAL OF OCEAN UNIVERSITY OF CHINA 13.6(2014):941-950.