Sperm cryopreservation in different grouper subspecies and application in interspecific hybridization

doi:10.1016/j.theriogenology.2015.12.023

	Sperm cryopreservation in different grouper subspecies and application in interspecific hybridization
	Liu, Q. H.1,2; Xiao, Z. Z.1,2; Wang, X. Y.1; Xu, S. H.1; Guan, S. G.3; Xu, C. A.4; Zhang, H. S.5; Li, J.1,2
刊名	THERIOGENOLOGY
	2016-05
卷号	85 期号:8 页码:1399-1407
关键词	Sperm Cryopreservation Interspecific hybridization Application Groupers
ISSN号	0093-691X
DOI	10.1016/j.theriogenology.2015.12.023
英文摘要	The present study established and confirmed an efficient technology for groupers: giant grouper Epinephelus lanceolatus, orange-spotted grouper E. coioides, seven-band grouper, E. septemfasciatus, and kelp grouper E. moara sperm cryopreservation and successfully applied the cryopreserved E. lanceolatus sperm into interspecific hybridization with E. coioides. For both E. lanceolatus and E. coioides, the best motility of postthaw sperm were achieved using 6% to 10% DMSO, 6% to 16% propylene glycol, and 6% ethylene glycol as cryoprotectants. Furthermore, we have successfully applied this method into the other two species of E. septemfasciatus (74.56 +/- 5.45%) and E. moara (71.67 +/- 5.10%) sperm cryopreservation and obtained high motility, respectively. Computer-assisted sperm motion analysis showed that the postthaw sperm of the four species of grouper could keep 30 to 35 minutes motile state in nature seawater. And the freezing thawing process decreased the sperm motility, speed, and longevity but did not significantly change the sperm movement pattern, and the progressive linear motion still was the dominant movement pattern. For the four species of grouper, the ultrastructural analysis showed 70% to 80% of the spermatozoa had intact morphology with a little of swelling; 20% to 30% were damaged, such as swelling or rupture of head, midpiece, and tail region; and 10% to 20% were severely damaged. Whereas, by the microscopic observation, more than 90% of the postthaw sperm presented normal morphology. In the artificial insemination and hybridization experiment, high fertilization rates and hatching rates were achieved when using 10% DMSO (88.7 +/- 5.3%, 85.3 +/- 7.4%) and 10% propylene glycol (86.8 +/- 3.3%, 83.1 +/- 6.6%), with no significant difference in comparison with control (92.2 +/- 1.4%, 87.9 +/- 4.2%). In addition, we found the embryos from postthaw sperm of E. lanceolatus and E. coioides eggs developed and grew normally as reported in previous study on hybridization of groupers (E. coioides x E. lanceolatus) using cryopreserved sperm. The results of the present study further validated the safety of the cryopreserved sperm in breeding production by assessing the fertilization capacity, embryo development, and larval growth. (C) 2016 Elsevier Inc. All rights reserved.
资助项目	earmarked fund for Modern Agro-industry Technology Research System[nycytx-50]
WOS研究方向	Reproductive Biology ; Veterinary Sciences
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000373968100006
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3457]
专题	自然资源部第一海洋研究所
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China; 3.Marine Biol Inst Shandong Prov, Qingdao, Peoples R China; 4.State Ocean Adm, Inst Oceanog 3, Engn Technol Res Ctr Comprehens Utilizat Biol Res, Qingdao, Peoples R China; 5.Qingdao Tongyong Aquaculture Ltd Co, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Q. H.,Xiao, Z. Z.,Wang, X. Y.,et al. Sperm cryopreservation in different grouper subspecies and application in interspecific hybridization[J]. THERIOGENOLOGY,2016,85(8):1399-1407.
APA	Liu, Q. H..,Xiao, Z. Z..,Wang, X. Y..,Xu, S. H..,Guan, S. G..,...&Li, J..(2016).Sperm cryopreservation in different grouper subspecies and application in interspecific hybridization.THERIOGENOLOGY,85(8),1399-1407.
MLA	Liu, Q. H.,et al."Sperm cryopreservation in different grouper subspecies and application in interspecific hybridization".THERIOGENOLOGY 85.8(2016):1399-1407.