Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea) by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

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	Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea) by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry
	Yang, HX (Yang Hong-Xia)5; Liu, W (Liu Wei); Li, B (Li Bing); Wei, W (Wei Wei); Zhang, HJ (Zhang Hui-Juan); Chen, DY (Chen Deng-Yun)
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2009
卷号	37 期号:10 页码:1511-1514
关键词	Speciation analysis cadmium size exclusion chromatography-high performance liquid chromatography inductively coupled plasma mass spectrometry
ISSN号	0253-3820
英文摘要	The method based on the coupling of size-exclusion HPLC with ICP-MS was used for the speciation analysis of cadmium binding non-protein thiols in extracts of Indian mustard(Brassica Juncea). In the presence of Cd stress, phytochalatins (PCs) were induced, and Cd ions in extracts were associated with the induced PCs. So four different Cd species, PC3-Cd, PC2-Cd, glutathione (GSH)-Cd and Cys-Cd, were detected in the samples, which demonstrated that PCs were enzymatically synthesized from glutathione (GSH, Glu-Cys-Gly) by the constitutive enzyme PC synthase. The results indicated that GSH-Cd exists as the main species in leaf samples while PC2-Cd is the main species in root samples. A non-oxidizing(nitrogen) preparation environment and a -70 degrees C preservation condition were employed to avoid the oxidation of sulfhydryl groups.
学科主题	岩矿鉴定与测试
WOS关键词	PHYTOCHELATINS ; ACCUMULATION ; ROOTS
WOS研究方向	Chemistry
语种	中文
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000271686300021
内容类型	期刊论文
源URL	[http://119.78.100.197/handle/2HKVOGP0/38909]
专题	中国地质调查局
作者单位	1.[Yang Hong-Xia 2.Liu Wei 3.Natl Res Ctr Geoanal, Beijing 100037, Peoples R China [Wei Wei 4.Agilent Technol, Beijing 100022, Peoples R China 5.中国地质科学院国家地质实验测试中心
推荐引用方式 GB/T 7714	Yang, HX ,Liu, W ,Li, B ,et al. Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea) by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(10):1511-1514.
APA	Yang, HX ,Liu, W ,Li, B ,Wei, W ,Zhang, HJ ,&Chen, DY .(2009).Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea) by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(10),1511-1514.
MLA	Yang, HX ,et al."Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea) by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.10(2009):1511-1514.