A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian   Fluid Behavior of Zebrafish Blood at High Shear Rates

doi:10.1038/s41598-017-02253-7

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	A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
	Lee, Juhyun ; Chou, Tzu-Chieh ; Kang, Dongyang ; Kang, Hanul ; Chen, Junjie ; Baek, Kyung In ; Wang, Wei ; Ding, Yichen ; Di Carlo, Dino ; Tai, Yu-Chong ; Hsiai, Tzung K.
刊名	SCIENTIFIC REPORTS
	2017
关键词	ADULT ZEBRAFISH VISCOMETER VISCOSITY MICROSCOPY REPAIR MODEL
DOI	10.1038/s41598-017-02253-7
英文摘要	Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3 mu L) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (> 500 s(-1)), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s(-1) were closely correlated at 27 degrees C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.; National Institutes of Health (NIH) [HL118650, HL083015, HL111437, HL129727]; American Heart Association (AHA) Pre-Doctoral Fellowship [15PRE21400019]; SCI(E); ARTICLE; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473392]
专题	工学院
推荐引用方式 GB/T 7714	Lee, Juhyun,Chou, Tzu-Chieh,Kang, Dongyang,et al. A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates[J]. SCIENTIFIC REPORTS,2017.
APA	Lee, Juhyun.,Chou, Tzu-Chieh.,Kang, Dongyang.,Kang, Hanul.,Chen, Junjie.,...&Hsiai, Tzung K..(2017).A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates.SCIENTIFIC REPORTS.
MLA	Lee, Juhyun,et al."A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates".SCIENTIFIC REPORTS (2017).