A bilaminated decellularized scaffold for islet transplantation:   Structure, properties and functions in diabetic mice

doi:10.1016/j.biomaterials.2017.05.033

CORC > 北京大学 > 工学院

	A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice
	Wang, Xi ; Wang, Kai ; Zhang, Wei ; Qiang, Ming ; Luo, Ying
刊名	BIOMATERIALS
	2017
关键词	Type I diabetes Decellularized material Islet Transplantation Hyperglycemia POLYMER SCAFFOLDS ENGRAFTMENT MEMBRANE RELEASE MOUSE SITE
DOI	10.1016/j.biomaterials.2017.05.033
英文摘要	Ectopic transplantation of islets provides a beta cell-replacement approach that may allow the recovery of physiological regulation of the blood sugar level in patients with Type I diabetes (T1D). In development of new extrahepatic islet transplantation protocols in support of the islet engraftment, it is pivotal to develop scaffold materials with multifaceted functions to provide beneficial microenvironment, mediate host response in favor of vascularization/islet integration and maintain long-term islet function at the transplantation site. In this study, a new composite bilaminar decellularized scaffold (CDS) was fabricated with differential structural, degradation and mechanical properties by the combination of a fast-degrading porous collagen matrix and a mechanically supportive porcine pericardium. When investigated in the epididymal fat pad in syngeneic mouse models, it was shown that CDS could serve as superior scaffolds to promote islet adhesion and viability, and islet-CDS constructs also allowed rapid reversal of the hyperglycemic condition in the host. The engraftment and effects of islets were achieved at low islet numbers, accompanied by minimal adverse tissue reactions and optimal islet integration with the surrounding fat tissue. The bioactive surface, mechanical/chemical durability and biocompatibility of the CDS may all have played important roles in facilitating the engraftment of islets. Our study provided new insights into scaffold's function in the interplay of cells, materials and host tissue and the extracellular matrix-based scaffolds have potential for clinical translation in the beta cell-replacement therapy to treat T1D. (C) 2017 Elsevier Ltd. All rights reserved.; National Natural Science Foundation of China [31322021]; National Key Research and Development Program of China [2016YFC1101301]; SCI(E); ARTICLE; 80-90; 138
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471318]
专题	工学院
推荐引用方式 GB/T 7714	Wang, Xi,Wang, Kai,Zhang, Wei,et al. A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice[J]. BIOMATERIALS,2017.
APA	Wang, Xi,Wang, Kai,Zhang, Wei,Qiang, Ming,&Luo, Ying.(2017).A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice.BIOMATERIALS.
MLA	Wang, Xi,et al."A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice".BIOMATERIALS (2017).