Effect of follicular dendritic cell secreted protein on gene expression   of human periodontal ligament cells

doi:10.1016/j.archoralbio.2017.05.005

CORC > 北京大学 > 工学院

	Effect of follicular dendritic cell secreted protein on gene expression of human periodontal ligament cells
	Xiang, Lin ; Xin, Na ; Yuan, Ying ; Hou, Xiaogang ; Chen, Junwei ; Wei, Na ; Gong, Ping
刊名	ARCHIVES OF ORAL BIOLOGY
	2017
关键词	Follicular dendritic cell secreted protein (FDC-SP) Lentiviral vector Human periodontal ligament cells (hPDLCs) Microarray analysis Differential gene expression KINASE INHIBITOR P21 GROWTH FACTOR-I FDC-SP STEM-CELLS T-CELLS IDENTIFICATION PROLIFERATION FIBROBLASTS REGULATOR RESPONSES
DOI	10.1016/j.archoralbio.2017.05.005
英文摘要	Objective: The objective of this study was to investigate the specific roles of follicular dendritic cell secreted protein (FDC-SP), a protein exists in saliva, in the inhibition of calcium precipitation during periodontal regeneration, as well as affect phenotype expression of human periodontal ligament cells (hPDLCs) during the differentiation process. Design: To investigate this, we applied microarray technology to identify gene expression changes in hPDLCs transfected with FDC-SP and then clustered them according to their biological functions. Results: One hundred seventy-one genes were found differentially expressed by at least two-fold between FDC-SP-transfected and empty vector-transfected cells. Besides, genes encoding cell-cycle proteins, blood-related and cell differentiation-related proteins tended to be up-regulated after FDC-SP transfection, whereas cytokine/growth factors, signal transduction and metabolism-related genes tended to be down-regulated in hPDLCs overexpression FDC-SP. Conclusions: The present study investigated FDC-SP's roles in hPDLCs' phenotype expression, via comparing the gene expression profiles between FDC-SP-transfected hPDLCs and empty vector-transfected cells upon microarray analysis. hPDLCs overexpression FDC-SP appear to display different gene expression patterns. In all, these observations showed a potential of FDC-SP in the maintenance of PDL homeostasis and its ultimate contribution to periodontal would-healing processes.; National Natural Science Foundation of China [81000443, 81400522]; Youth Science Foundation of West China Hospital of Stomatology [2016-11]; SCI(E); ARTICLE; 151-159; 81
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471273]
专题	工学院
推荐引用方式 GB/T 7714	Xiang, Lin,Xin, Na,Yuan, Ying,et al. Effect of follicular dendritic cell secreted protein on gene expression of human periodontal ligament cells[J]. ARCHIVES OF ORAL BIOLOGY,2017.
APA	Xiang, Lin.,Xin, Na.,Yuan, Ying.,Hou, Xiaogang.,Chen, Junwei.,...&Gong, Ping.(2017).Effect of follicular dendritic cell secreted protein on gene expression of human periodontal ligament cells.ARCHIVES OF ORAL BIOLOGY.
MLA	Xiang, Lin,et al."Effect of follicular dendritic cell secreted protein on gene expression of human periodontal ligament cells".ARCHIVES OF ORAL BIOLOGY (2017).