RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances   triacylglycerol biosynthesis in the oleaginous marine alga   Nannochloropsis salina

doi:10.1038/s41598-017-11932-4

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	RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina
	Ma, Xiaonian ; Yao, Lina ; Yang, Bo ; Lee, Yuan Kun ; Chen, Feng ; Liu, Jin
刊名	SCIENTIFIC REPORTS
	2017
关键词	EFFICIENCY NUCLEAR TRANSFORMATION NITROGEN STARVATION BRASSICA-NAPUS FATTY-ACID MICROALGAE OCEANICA REVEALS LIGHT PURIFICATION OIL
DOI	10.1038/s41598-017-11932-4
英文摘要	Oleaginous microalgae have been emerging as the third-generation feedstocks for biofuel production. Genetic manipulation for improving triacylglycerol (TAG) accumulation represents a promising approach towards the economics of microalgal biofuels. Acetyl-CoA, the essential carbon precursor for de novo fatty acid biosynthesis, can be derived from pyruvate catalyzed by pyruvate dehydrogenase, which is negatively regulated by pyruvate dehydrogenase kinase (PDK). In the present study, we characterized a PDK gene (NsPDK) from Nannochloropsis salina. Subcellular localization assay assisted by green fluorescence protein (GFP) fusion indicated the localization of NsPDK in mitochondria of N. salina cells. NsPDK knockdown via RNA interference strategy attenuated NsPDK expression at the mRNA level and its enzymatic activity in vivo, leading to faster TAG accumulation without compromising cell growth under high light stress conditions. Interestingly, the TAG increase was accompanied by a decline in membrane polar lipids. NsPDK knockdown also altered fatty acid profile in N. salina. Furthermore, transcriptional analysis suggested that the carbon metabolic pathways might be influenced by NsPDK knockdown leading to diverted carbon flux towards TAG synthesis. Taken together, our results demonstrate the role of NsPDK in regulating TAG accumulation and provide valuable insights into future manipulation of oleaginous microalgae for improving biofuel production.; National Natural Science Foundation of China [31471717, 31571807]; Ministry of Science and Technology of China [2012AA02A707]; Singapore-Peking University Research Centre for a Sustainable Low-carbon Future; SCI(E); ARTICLE; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470884]
专题	工学院
推荐引用方式 GB/T 7714	Ma, Xiaonian,Yao, Lina,Yang, Bo,et al. RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina[J]. SCIENTIFIC REPORTS,2017.
APA	Ma, Xiaonian,Yao, Lina,Yang, Bo,Lee, Yuan Kun,Chen, Feng,&Liu, Jin.(2017).RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina.SCIENTIFIC REPORTS.
MLA	Ma, Xiaonian,et al."RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina".SCIENTIFIC REPORTS (2017).