Substrate effect modulates adhesion and proliferation of fibroblast on   graphene layer

doi:10.1016/j.colsurfb.2016.07.008

CORC > 北京大学 > 工学院

	Substrate effect modulates adhesion and proliferation of fibroblast on graphene layer
	Lin, Feng ; Du, Feng ; Huang, Jianyong ; Chau, Alicia ; Zhou, Yongsheng ; Duan, Huiling ; Wang, Jianxiang ; Xiong, Chunyang
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2016
关键词	Graphene Cytocompatibility Cell function and behavior Substrate stiffness Surface wetting property MESENCHYMAL STEM-CELLS OSTEOGENIC DIFFERENTIATION STIFFNESS GRADIENTS MATRIX-RIGIDITY FOCAL ADHESION SOFT ELASTICITY FORCE NUCLEATION BEHAVIOR
DOI	10.1016/j.colsurfb.2016.07.008
英文摘要	Graphene is an emerging candidate for biomedical applications, including biosensor, drug delivery and scaffold biomaterials. Cellular functions and behaviors on different graphene-coated substrates, however, still remain elusive to a great extent. This paper explored the functional responses of cells such as adhesion and proliferation, to different kinds of substrates including coverslips, silicone, polydimethylsiloxane (PDMS) with different curing ratios, PDMS treated with oxygen plasma, and their counterparts coated with single layer graphene (SLG). Specifically, adherent cell number, spreading area and cytoskeleton configuration were exploited to characterize cell-substrate adhesion ability, while MTT assay was employed to test the proliferation capability of fibroblasts. Experimental outcome demonstrated graphene coating had excellent cytocompatibility, which could lead to an increase in early adhesion, spreading, proliferation, and remodeling of cytoskeletons of fibroblast cells. Notably, it was found that the underlying substrate effect, e.g., stiffness of substrate materials, could essentially regulate the adhesion and proliferation of cells cultured on graphene. The stiffer the substrates were, the stronger the abilities of adhesion and proliferation of fibroblasts were. This study not only deepens our understanding of substrate-modulated interfacial interactions between live cells and graphene, but also provides a valuable guidance for the design and application of graphene-based biomaterials in biomedical engineering. (C) 2016 Elsevier B.V. All rights reserved.; National Basic Research Program of China [2013CB933702]; National Natural Science Foundation of China (NSFC) [11272015, 11472013, 11225208]; Peking University 985 program; SCI(E); EI; PubMed; ARTICLE; jxwang@pku.edu.cn; cyxiong@pku.edu.cn; 785-793; 146
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/447901]
专题	工学院
推荐引用方式 GB/T 7714	Lin, Feng,Du, Feng,Huang, Jianyong,et al. Substrate effect modulates adhesion and proliferation of fibroblast on graphene layer[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2016.
APA	Lin, Feng.,Du, Feng.,Huang, Jianyong.,Chau, Alicia.,Zhou, Yongsheng.,...&Xiong, Chunyang.(2016).Substrate effect modulates adhesion and proliferation of fibroblast on graphene layer.COLLOIDS AND SURFACES B-BIOINTERFACES.
MLA	Lin, Feng,et al."Substrate effect modulates adhesion and proliferation of fibroblast on graphene layer".COLLOIDS AND SURFACES B-BIOINTERFACES (2016).