Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by   conjugating with gold nanoparticles and its in vivo bioluminescence   tomographic evaluation

doi:10.1016/j.biomaterials.2016.02.007

CORC > 北京大学 > 工学院

	Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by conjugating with gold nanoparticles and its in vivo bioluminescence tomographic evaluation
	Ma, Xibo ; Hui, Hui ; Jin, Yushen ; Dong, Di ; Liang, Xiaolong ; Yang, Xin ; Tan, Ke ; Dai, Zhifei ; Cheng, Zhen ; Tian, Jie
刊名	BIOMATERIALS
	2016
关键词	Antibody therapy Gold nanoparticles Hepatocellular carcinoma Cell proliferation Bioluminescence tomography POSITRON-EMISSION-TOMOGRAPHY TUMOR-IMMUNOTHERAPY MONOCLONAL-ANTIBODY LIVER-CANCER CELL-CYCLE DELIVERY APPLICATIONS TARGETED THERAPIES LUNG-CANCER EXPRESSION APOPTOSIS
DOI	10.1016/j.biomaterials.2016.02.007
英文摘要	SM5-1 is a humanized mouse monoclonal antibody, targeting an over-expressed membrane protein of approximately 230 kDa in hepatocellular carcinoma (HCC). SM5-1 can be used for target therapy in hepatocellular carinoma due to its ability of inhibiting cell growth and inducing apoptosis. However, the tumor inhibition efficacy of SM5-1 in HCC cancer treatment remains low. In this study, we synthesized SM5-1-conjugated gold nanoparticles (Au-SM5-1 NPs) and investigated their anticancer efficacy in HCC both in vitro and in vivo. The tumor inhibition rates of Au-SM5-1 NPs for subcutaneous tumor mice were 40.10% +/- 4.34%, 31.37% +/- 5.12%, and 30.63% +/- 4.87% on day 12, 18, and 24 post-treatment as determined by bioluminescent intensity. In addition, we investigated the antitumor efficacy of Au-SM5-1 NPs in orthotopic HCC tumor models. The results showed that the inhibition rates of Au-SM5-1 NPs can reach up to 39.64% +/- 4.87% on day 31 post-treatment determined by the bioluminescent intensity of the abdomen in tumor-bearing mice. Furthermore, three-dimensional reconstruction results of the orthotopic tumor revealed that Au-SM5-1 NPs significantly inhibited tumor growth compared with SM5-1 alone. Our results suggested that the developed Au-SM5-1 NPs has great potential as an antibody based nano-drug for HCC therapy. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licensesiby-nc-nd/4.0/).; National Natural Science Foundation of China [81227901, 61231004, 81471739, 81527805, 81301346]; Key Research Program of the Chinese Academy of Sciences [KGZD-EW-T03]; Scientific Research and Equipment Development Project of Chinese Academy of Sciences [YZ201457]; National Basic Research Program of China (973 Program) [2011CB707700]; SCI(E); EI; PubMed; ARTICLE; zcheng@stanford.edu; jie.tian@ia.ac.cn; 46-56; 87
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434811]
专题	工学院
推荐引用方式 GB/T 7714	Ma, Xibo,Hui, Hui,Jin, Yushen,et al. Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by conjugating with gold nanoparticles and its in vivo bioluminescence tomographic evaluation[J]. BIOMATERIALS,2016.
APA	Ma, Xibo.,Hui, Hui.,Jin, Yushen.,Dong, Di.,Liang, Xiaolong.,...&Tian, Jie.(2016).Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by conjugating with gold nanoparticles and its in vivo bioluminescence tomographic evaluation.BIOMATERIALS.
MLA	Ma, Xibo,et al."Enhanced immunotherapy of SM5-1 in hepatocellular carcinoma by conjugating with gold nanoparticles and its in vivo bioluminescence tomographic evaluation".BIOMATERIALS (2016).