MiR-19a regulates PTEN expression to mediate glycogen synthesis in   hepatocytes

doi:10.1038/srep11602

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	MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes
	Dou, Lin ; Meng, Xiangyu ; Sui, Xiaofang ; Wang, Shuyue ; Shen, Tao ; Huang, Xiuqing ; Guo, Jun ; Fang, Weiwei ; Man, Yong ; Xi, Jianzhong ; Li, Jian
刊名	SCIENTIFIC REPORTS
	2015
关键词	HEPATIC INSULIN-RESISTANCE MIR-17-92 CLUSTER TUMOR-SUPPRESSOR INTERLEUKIN-6 MICRORNAS MECHANISM CELLS
DOI	10.1038/srep11602
英文摘要	MiR-19a, a member of mir-17-92 microRNA clusters, has been demonstrated to promote cell proliferation and angiogenesis via regulating the PI3K/AKT pathway, the major insulin signaling pathway. However, whether miR-19a plays an important role in glycogen synthesis in hepatocytes remains unknown. Here, we define the impact of miR-19a on glycogen synthesis and IL-6-induced reduced glycogenesis in hepatocytes and its underlying mechanisms. Our studies indicate that miR19a was down-regulated in the livers of db/db mice and mice injected with IL-6, as well as mouse NCTC 1469 hepatocytes and HEP 1-6 hepatocytes treated by IL-6. We found that over-expression of miR-19a in NCTC 1469 cells and HEP 1-6 cells led to increased activation of the AKT/GSK pathway and synthesis of glycogen, whereas down-regulation of miR-19a impaired AKT/GSK phosphorylation and glycogenesis. Over-expression of miR-19a ameliorated IL-6-induced reduced glycogen synthesis in hepatocytes. Moreover, we identified PTEN as the target of miR-19a by a luciferase assay. Down-regulation of PTEN rescued the effects of miR-19a suppression on the activation of the AKT/GSK pathway and improved glycogenesis in NTC 1469 cells. These findings show for the first time that miR-19a might activate the AKT/GSK pathway and glycogenesis via down-regulation of PTEN expression.; National Basic Research Program of China [2012CB517502, 2014CB910503]; National Natural Science Foundation of China [81070634, 81270887]; SCI(E); PubMed; ARTICLE; jzxi@pku.edu.cn; lijian@bjhmoh.cn; 11602; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/419129]
专题	工学院
推荐引用方式 GB/T 7714	Dou, Lin,Meng, Xiangyu,Sui, Xiaofang,et al. MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes[J]. SCIENTIFIC REPORTS,2015.
APA	Dou, Lin.,Meng, Xiangyu.,Sui, Xiaofang.,Wang, Shuyue.,Shen, Tao.,...&Li, Jian.(2015).MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes.SCIENTIFIC REPORTS.
MLA	Dou, Lin,et al."MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes".SCIENTIFIC REPORTS (2015).