Three-dimensional multimodal sub-diffraction imaging with spinning-disk   confocal microscopy using blinking/fluctuating probes

doi:10.1007/s12274-015-0736-8

CORC > 北京大学 > 工学院

	Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes
	Chen, Xuanze ; Zeng, Zhiping ; Wang, Hening ; Xi, Peng
刊名	NANO RESEARCH
	2015
关键词	multi-modality super-resolution microscopy three-dimensional spinning-disk confocal SUPERRESOLUTION FLUORESCENCE MICROSCOPY PHOTOACTIVATED LOCALIZATION MICROSCOPY STRUCTURED-ILLUMINATION MICROSCOPY OPTICAL RECONSTRUCTION MICROSCOPY QUANTUM DOTS STIMULATED-EMISSION LIVE CELLS RESOLUTION NANOSCOPY PROTEIN
DOI	10.1007/s12274-015-0736-8
英文摘要	Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk (SD) confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD confocal microscopy, such as fast imaging speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along all three dimensions. Both axial and lateral resolution can be improved simultaneously by virtue of the blinking/fluctuating nature of modified fluorescent probes, exemplified with the quantum dots. Further, super-resolution images with dual modality can be obtained through super-resolution optical fluctuation imaging (SOFI) and bleaching/blinking-assisted localization microscopy (BaLM). Therefore, fast super-resolution imaging can be achieved with SD-SOFI by capturing only 100 frames while SD-BaLM yields high-resolution imaging.; National Instrumentation Program [2013YQ03065102]; National Basic Research Program of China [2011CB809101, 2010CB933901]; National Natural Science Foundation of China [61178076, 31327901, 61475010]; SCI(E); 中国科学引文数据库(CSCD); ARTICLE; xipeng@pku.edu.cn; 7; 2251-2260; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/418832]
专题	工学院
推荐引用方式 GB/T 7714	Chen, Xuanze,Zeng, Zhiping,Wang, Hening,et al. Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes[J]. NANO RESEARCH,2015.
APA	Chen, Xuanze,Zeng, Zhiping,Wang, Hening,&Xi, Peng.(2015).Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes.NANO RESEARCH.
MLA	Chen, Xuanze,et al."Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes".NANO RESEARCH (2015).