Xylose fermentation efficiency and inhibitor tolerance of the   recombinant industrial Saccharomyces cerevisiae strain NAPX37

doi:10.1007/s00253-015-7167-z

CORC > 北京大学 > 工学院

	Xylose fermentation efficiency and inhibitor tolerance of the recombinant industrial Saccharomyces cerevisiae strain NAPX37
	Li, Yun-Cheng ; Mitsumasu, Kanako ; Gou, Zi-Xi ; Gou, Min ; Tang, Yue-Qin ; Li, Guo-Ying ; Wu, Xiao-Lei ; Akamatsu, Takashi ; Taguchi, Hisataka ; Kida, Kenji
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2016
关键词	Industrial Saccharomyces cerevisiae Xylose fermentation Lignocellulosic bioethanol Inhibitor tolerance Batch fermentation Continuous fermentation IMPROVES ETHANOL-PRODUCTION COENZYME PREFERENCE ACID HYDROLYSIS PICHIA-STIPITIS REDUCTASE YEAST PATHWAY PERSPECTIVES GENERATION CONVERSION
DOI	10.1007/s00253-015-7167-z
英文摘要	Industrial yeast strains with good xylose fermentation ability and inhibitor tolerance are important for economical lignocellulosic bioethanol production. The flocculating industrial Saccharomyces cerevisiae strain NAPX37, harboring the xylose reductase-xylitol dehydrogenase (XR-XDH)-based xylose metabolic pathway, displayed efficient xylose fermentation during batch and continuous fermentation. During batch fermentation, the xylose consumption rates at the first 36 h were similar (1.37 g/L/h) when the initial xylose concentrations were 50 and 75 g/L, indicating that xylose fermentation was not inhibited even when the xylose concentration was as high as 75 g/L. The presence of glucose, at concentrations of up to 25 g/L, did not affect xylose consumption rate at the first 36 h. Strain NAPX37 showed stable xylose fermentation capacity during continuous ethanol fermentation using xylose as the sole sugar, for almost 1 year. Fermentation remained stable at a dilution rate of 0.05/h, even though the xylose concentration in the feed was as high as 100 g/L. Aeration rate, xylose concentration, and MgSO4 concentration were found to affect xylose consumption and ethanol yield. When the xylose concentration in the feed was 75 g/L, a high xylose consumption rate of 6.62 g/L/h and an ethanol yield of 0.394 were achieved under an aeration rate of 0.1 vvm, dilution rate of 0.1/h, and 5 mM MgSO4. In addition, strain NAPX37 exhibited good tolerance to inhibitors such as weak acids, furans, and phenolics during xylose fermentation. These findings indicate that strain NAPX37 is a promising candidate for application in the industrial production of lignocellulosic bioethanol.; National Natural Science Foundation of China [31170093]; SCI(E); EI; PubMed; ARTICLE; tangyq@scu.edu.cn; 3; 1531-1542; 100
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416531]
专题	工学院
推荐引用方式 GB/T 7714	Li, Yun-Cheng,Mitsumasu, Kanako,Gou, Zi-Xi,et al. Xylose fermentation efficiency and inhibitor tolerance of the recombinant industrial Saccharomyces cerevisiae strain NAPX37[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016.
APA	Li, Yun-Cheng.,Mitsumasu, Kanako.,Gou, Zi-Xi.,Gou, Min.,Tang, Yue-Qin.,...&Kida, Kenji.(2016).Xylose fermentation efficiency and inhibitor tolerance of the recombinant industrial Saccharomyces cerevisiae strain NAPX37.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY.
MLA	Li, Yun-Cheng,et al."Xylose fermentation efficiency and inhibitor tolerance of the recombinant industrial Saccharomyces cerevisiae strain NAPX37".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2016).