CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic   Electrodes

doi:10.1515/ijnsns-2012-0400

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	CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes
	Wang, Shanshan ; Wei, Qiong ; Zhu, Tao ; Huang, Jianyong ; Yu, Min ; Sha, Yinlin ; Xiong, Chunyang ; Fang, Jing
刊名	international journal of nonlinear sciences and numerical simulation
	2012
关键词	CD4+T cell cell counting hydrodynamic focusing fluidic electrodes microfluidics MICRO-COULTER COUNTER MICROFLUIDIC DEVICE BACTERIAL-CELLS AC-IMPEDANCE SPECTROSCOPY BIOSENSOR
DOI	10.1515/ijnsns-2012-0400
英文摘要	A practical label-free method for counting CD4+ T cells is proposed on the basis of a microfluidic chip with fluidic electrodes. With the help of hydrodynamic focusing, two sheath flows of KCl solution, serving as electric conductors to replace solid metal electrodes, are used to squeeze the cell suspension. By measuring the electrical impedances between the fluidic electrodes, a linear relationship is found between the logarithmic value of cell concentration and the impedance value (R-2 = 0.97). The detection limit of CD4+ T lymphocytes reaches as low as similar to 10 cells.mu L-1, Both the CD4+ T cell concentration of the nude mice group and that of the normal group were counted to further test the function of the chip system, which indicated that there existed significant differences between these groups, showing the approach is valid for identifying immunodeficiency.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000310334900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Multidisciplinary; Mathematics, Applied; Mechanics; Physics, Mathematical; SCI(E); 1; ARTICLE; 5; 311-317; 13
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/327122]
专题	工学院
推荐引用方式 GB/T 7714	Wang, Shanshan,Wei, Qiong,Zhu, Tao,et al. CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes[J]. international journal of nonlinear sciences and numerical simulation,2012.
APA	Wang, Shanshan.,Wei, Qiong.,Zhu, Tao.,Huang, Jianyong.,Yu, Min.,...&Fang, Jing.(2012).CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes.international journal of nonlinear sciences and numerical simulation.
MLA	Wang, Shanshan,et al."CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes".international journal of nonlinear sciences and numerical simulation (2012).