Bacteria in Crude Oil Survived Autoclaving and Stimulated Differentially   by Exogenous Bacteria

doi:10.1371/journal.pone.0040842

CORC > 北京大学 > 工学院

	Bacteria in Crude Oil Survived Autoclaving and Stimulated Differentially by Exogenous Bacteria
	Gong, Xiao-Cui ; Liu, Ze-Shen ; Guo, Peng ; Chi, Chang-Qiao ; Chen, Jian ; Wang, Xing-Biao ; Tang, Yue-Qin ; Wu, Xiao-Lei ; Liu, Chun-Zhong
刊名	plos one
	2012
关键词	TEMPERATURE PETROLEUM RESERVOIRS N-ALKANE DEGRADATION MICROBIAL COMMUNITIES HEAT-RESISTANCE SEQUENCE DATA DIESEL FUEL MICROORGANISMS STRAIN STRESS SOIL
DOI	10.1371/journal.pone.0040842
英文摘要	Autoclaving of crude oil is often used to evaluate the hydrocarbon-degrading abilities of bacteria. This may be potentially useful for bioaugmentation and microbial enhanced oil recovery (MEOR). However, it is not entirely clear if "endogenous" bacteria (e.g., spores) in/on crude oil survive the autoclaving process, or influence subsequent evaluation of the hydrocarbon-degradation abilities of the "exogenous" bacterial strains. To test this, we inoculated autoclaved crude oil medium with six exogenous bacterial strains (three Dietzia strains, two Acinetobacter strains, and one Pseudomonas strain). The survival of the spore-forming Bacillus and Paenibacillus and the non-spore-forming mesophilic Pseudomonas, Dietzia, Alcaligenes, and Microbacterium was detected using a 16S rRNA gene clone library and terminal restriction fragment length polymorphism (T-RFLP) analysis. However, neither bacteria nor bacterial activity was detected in three controls consisting of non-inoculated autoclaved crude oil medium. These results suggest that detection of endogenous bacteria was stimulated by the six inoculated strains. In addition, inoculation with Acinetobacter spp. stimulated detection of Bacillus, while inoculation with Dietzia spp. and Pseudomonas sp. stimulated the detection of more Pseudomonas. In contrast, similar exogenous bacteria stimulated similar endogenous bacteria at the genus level. Based on these results, special emphasis should be applied to evaluate the influence of bacteria capable of surviving autoclaving on the hydrocarbon-degrading abilities of exogenous bacteria, in particular, with regard to bioaugmentation and MEOR. Bioaugmentation and MEOR technologies could then be developed to more accurately direct the growth of specific endogenous bacteria that may then improve the efficiency of treatment or recovery of crude oil.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309742800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 6; ARTICLE; 9; e40842; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315673]
专题	工学院
推荐引用方式 GB/T 7714	Gong, Xiao-Cui,Liu, Ze-Shen,Guo, Peng,et al. Bacteria in Crude Oil Survived Autoclaving and Stimulated Differentially by Exogenous Bacteria[J]. plos one,2012.
APA	Gong, Xiao-Cui.,Liu, Ze-Shen.,Guo, Peng.,Chi, Chang-Qiao.,Chen, Jian.,...&Liu, Chun-Zhong.(2012).Bacteria in Crude Oil Survived Autoclaving and Stimulated Differentially by Exogenous Bacteria.plos one.
MLA	Gong, Xiao-Cui,et al."Bacteria in Crude Oil Survived Autoclaving and Stimulated Differentially by Exogenous Bacteria".plos one (2012).