In Vitro Cytotoxicity of Calcium Silicate-containing Endodontic Sealers

doi:10.1016/j.joen.2014.09.012

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	In Vitro Cytotoxicity of Calcium Silicate-containing Endodontic Sealers
	Zhou, Hui-min ; Du, Tian-feng ; Shen, Ya ; Wang, Zhe-jun ; Zheng, Yu-feng ; Haapasalo, Markus
刊名	journal of endodontics
	2015
关键词	Calcium silicate-containing sealer cell attachment cytotoxicity flow cytonnetry mineral trioxide aggregate ROOT-CANAL SEALERS MINERAL TRIOXIDE AGGREGATE ANTIBACTERIAL ACTIVITY SEALING ABILITY LONG-TERM REPAIR MTA BIOCOMPATIBILITY PERCHA SYSTEM
DOI	10.1016/j.joen.2014.09.012
英文摘要	Introduction: The cytotoxicity of 2 novel calcium silicate containing endodontic sealers to human gingival fibroblasts was studied. Methods,: EndoSequence BC (Brasseler, Savannah, GA), MTA Fillapex (Angelus Industria de Produtos Odontologicos S/A, Londrina, PR, Brazil) and a control sealer (AH Plus; Dentsply DeTrey GmbH, Konstanz, Germany) were evaluated. Human gingival fibroblasts were incubated for 3 days both with the extracts from fresh and set materials in culture medium and cultured on the surface of the set materials in Dulbecco-modified Eagle medium. Fibroblasts cultured in Dulbecco-modified Eagle medium were used as a control group. Cytotoxicity was evaluated by flow cytometry, and the adhesion of the fibroblasts to the surface of the set materials was assessed using scanning electron microscopy. The data of cell cytotoxicity were analyzed statistically using a 1-way analysis of variance test at a significance level of P < .05. Results: Cells incubated with extracts from BC Sealer showed higher viabilities at all extract concentrations than cells incubated with extracts from freshly mixed AH Plus and fresh and set MTA Fillapex, esspecially for the high extract concentrations (1:2 and 1:8 dilutions). Extracts from set MTA Fillapex of 2 weeks and older were more cytotoxic than extracts from freshly mixed and 1-week-old cement. With extract concentrations of 1:32 and lower, MTA Fillapex was no longer cytotoxic. After setting, AH Plus was no longer cytotoxic, and the fibroblast cells grew on set AH Plus equally as well as on BC Sealer. Conclusions: BC Sealer and MTA Fillapex, the 2 calcium silicate containing endodontic sealers, exhibited different cytotoxicity to human gingival fibroblasts.; Dentistry, Oral Surgery & Medicine; SCI(E); PubMed; 2; ARTICLE; markush@dentistry.ubc.ca; 1; 56-61; 41
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188491]
专题	工学院
推荐引用方式 GB/T 7714	Zhou, Hui-min,Du, Tian-feng,Shen, Ya,et al. In Vitro Cytotoxicity of Calcium Silicate-containing Endodontic Sealers[J]. journal of endodontics,2015.
APA	Zhou, Hui-min,Du, Tian-feng,Shen, Ya,Wang, Zhe-jun,Zheng, Yu-feng,&Haapasalo, Markus.(2015).In Vitro Cytotoxicity of Calcium Silicate-containing Endodontic Sealers.journal of endodontics.
MLA	Zhou, Hui-min,et al."In Vitro Cytotoxicity of Calcium Silicate-containing Endodontic Sealers".journal of endodontics (2015).