Dextran-coated fluorapatite crystals doped with Yb(3+)/Ho(3+) for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in?vitro and in?vivo

doi:10.1016/j.biomaterials.2015.02.050

CORC > 北京大学 > 工学院

	Dextran-coated fluorapatite crystals doped with Yb(3+)/Ho(3+) for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in?vitro and in?vivo
	Hu Xiaoqing ; Zhu Jingxian ; Li Xiyu ; Zhang Xin ; Meng Qingyang ; Yuan Lan ; Zhang Jiying ; Fu Xin ; Duan Xiaoning ; Chen Haifeng ; Ao Yingfang
刊名	biomaterials
	2015
关键词	BMSCs,Chondrogenic differentiation,In?vivo imaging,Upconversion fluorescence
DOI	10.1016/j.biomaterials.2015.02.050
英文摘要	Upconversion fluorescent nanoparticles are becoming more widely used as imaging contrast agents, owing to their high resolution and penetration depth, and avoidance of tissue auto-fluorescence and photodamage to cells. Here, we synthesized upconversion fluorescent crystals from rare-earth Yb(3+) and Ho(3+) co-doped fluorapatite (FA:Yb(3+)/Ho(3+)) suitable for long-term tracking and monitoring cartilage development (chondrogenesis) in bone marrow mesenchymal stem cells (BMSCs) in?vitro and in?vivo. We initially determined the structure, morphology and luminescence of the products using X-ray powder diffraction, transmission electron microscopy and two-photon confocal microscopy. When excited at 980?nm, FA:Yb(3+)/Ho(3+) crystals exhibited distinct upconversion fluorescence peaks at 543?nm and 654?nm. We then conjugated FA:Yb(3+)/Ho(3+) crystals with dextran to enhance hydrophilicity, biocompatibility and cell penetration. Next, we employed the dextran-coated FA:Yb(3+)/Ho(3+) crystals in labeling and tracking chondrogenic differentiation processes in BMSCs stably expressing green fluorescent protein (BMSCs(GFP)) in?vitro and in?vivo. Labeled BMSCs(GFP) were shown to reproducibly exhibit chondrogenic differentiation potential in RT-PCR analysis, histological assessment and immunohistochemistry. We observed continuous luminescence from the FA:Yb(3+)/Ho(3+) upconversion crystals at 4 weeks and 12 weeks post transplantation in BMSCs(GFP), while GFP fluorescence in both control and crystal-treated groups significantly decreased at 12 weeks after BMSCs(GFP) transplantation. We therefore demonstrate the high biocompatibility and stability of FA:Yb(3+)/Ho(3+) crystals in tracking and monitoring BMSCs chondrogenesis in?vitro and in?vivo, highlighting their excellent cell labeling potential in cartilage tissue engineering.Copyright ? 2015 Elsevier Ltd. All rights reserved.; SCI(E); EI; PubMed; 0; ARTICLE; haifeng.chen@pku.edu.cn; aoyingfang@163.com; 441-51; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/161248]
专题	工学院
推荐引用方式 GB/T 7714	Hu Xiaoqing,Zhu Jingxian,Li Xiyu,et al. Dextran-coated fluorapatite crystals doped with Yb(3+)/Ho(3+) for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in?vitro and in?vivo[J]. biomaterials,2015.
APA	Hu Xiaoqing.,Zhu Jingxian.,Li Xiyu.,Zhang Xin.,Meng Qingyang.,...&Ao Yingfang.(2015).Dextran-coated fluorapatite crystals doped with Yb(3+)/Ho(3+) for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in?vitro and in?vivo.biomaterials.
MLA	Hu Xiaoqing,et al."Dextran-coated fluorapatite crystals doped with Yb(3+)/Ho(3+) for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in?vitro and in?vivo".biomaterials (2015).