Doping Hydroxylated Cationic Lipid into PEGylated Cerasome Boosts in   Vivo siRNA Transfection Efficacy

doi:10.1021/bc500414e

CORC > 北京大学 > 工学院

	Doping Hydroxylated Cationic Lipid into PEGylated Cerasome Boosts in Vivo siRNA Transfection Efficacy
	Li, Yanyan ; Zheng, Shuquan ; Liang, Xiaolong ; Jin, Yushen ; Wu, Yidi ; Bai, Huichen ; Liu, Renfa ; Dai, Zhifei ; Liang, Zicai ; Shi, Tiejun
刊名	生物共轭化学
	2014
关键词	GENE DELIVERY SYSTEMIC DELIVERY NONVIRAL VECTORS NONHUMAN-PRIMATES RNA INTERFERENCE CANCER-THERAPY NANOPARTICLES THERAPEUTICS BIOCOMPATIBILITY CHOLESTEROL
DOI	10.1021/bc500414e
英文摘要	The therapeutic application of small interfering RNA (siRNA) requires safe nanocarriers for specific and efficient delivery in vivo. Herein, PEGylated cationic cerasomes (PCCs) were fabricated by doping a cationic lipid with a hydroxyl group into nanohybrid cerasomes. Multiple properties of PCCs provide a solution to many of the limitations associated with current platforms for the delivery of siRNA. The polyorganosiloxane surface imparts PCCs with higher morphological stability than conventional liposomes. The PEGylation of the cationic cerasome could protect the cerasome nanoparticles from agglomeration and macrophage capture, reduce protein absorption, and consequently prolong the blood circulating time and enhance the siRNA delivery efficiency. In addition, incorporation of the lipid containing a hydroxyl group further facilitates endosome release. Moreover, PCCs were further used to transport siRNA into the cytosol primarily via endocytosis. When applied to systemic administration, PCCs have demonstrated effective delivery into the liver and preferential uptake by hepatocytes in mice, thereby leading to high siRNA gene-silencing activity. All these results show potential therapeutic applications of PCCs-mediated delivery of siRNA for liver diseases.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000345309000017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic; SCI(E); PubMed; 7; ARTICLE; zhifei.dai@pku.edu.cn; liangz@pku.edu.cn; 11; 2055-2066; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/153825]
专题	工学院
推荐引用方式 GB/T 7714	Li, Yanyan,Zheng, Shuquan,Liang, Xiaolong,et al. Doping Hydroxylated Cationic Lipid into PEGylated Cerasome Boosts in Vivo siRNA Transfection Efficacy[J]. 生物共轭化学,2014.
APA	Li, Yanyan.,Zheng, Shuquan.,Liang, Xiaolong.,Jin, Yushen.,Wu, Yidi.,...&Shi, Tiejun.(2014).Doping Hydroxylated Cationic Lipid into PEGylated Cerasome Boosts in Vivo siRNA Transfection Efficacy.生物共轭化学.
MLA	Li, Yanyan,et al."Doping Hydroxylated Cationic Lipid into PEGylated Cerasome Boosts in Vivo siRNA Transfection Efficacy".生物共轭化学 (2014).