Detection of heart failure-related biomarker in whole blood with   graphene field effect transistor biosensor

doi:10.1016/j.bios.2016.12.018

CORC > 北京大学 > 信息科学技术学院

	Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor
	Lei, Yong-Min ; Xiao, Meng-Meng ; Li, Yu-Tao ; Xu, Li ; Zhang, Hong ; Zhang, Zhi-Yong ; Zhang, Guo-Jun
刊名	BIOSENSORS & BIOELECTRONICS
	2017
关键词	Brain natriuretic peptide Reduced graphene oxide Platinum nanoparticles Human whole blood Field effect transistor biosensor Detection BRAIN NATRIURETIC PEPTIDE LABEL-FREE DETECTION CARDIAC BIOMARKER ENZYME-IMMUNOASSAY NT-PROBNP OXIDATION NANOCOMPOSITE HYBRIDIZATION NANOPARTICLE IMMUNOSENSOR
DOI	10.1016/j.bios.2016.12.018
英文摘要	Since brain natriuretic peptide (BNP) has become internationally recognized biomarkers in the diagnosis and prognosis of heart failure (HF), it is highly desirable to search for a novel sensing tool for detecting the patient's BNP level at the early stage. Here we report a platinum nanoparticles (PtNPs)-decorated reduced graphene oxide (rGO) field effect transistor (FET) biosensor coupled with a microfilter system for label-free and highly sensitive detection of BNP in whole blood. The PtNPs-decorated rGO FET sensor was obtained by drop-casting rGO onto the pre-fabricated FET chip and subsequently assembling PtNPs on the graphene surface. After antiBNP was bound to the PtNPs surface, BNP was successfully detected by the anti-BNP immobilized FET biosensor. It was found that the developed FET biosensor was able to achieve a low detection limitation of 100 fM. Moreover, BNP was successfully detected in human whole blood sample treated by a custom-made microfilter, suggesting the sensor's capability of working in a complex sample matrix. The developed FET biosensor provides a new sensing platform for protein detection, showing its potential applications in clinic sample.; National Natural Science Foundation of China [21275040, 21475034, 21675041, 61390504]; SCI(E); ARTICLE; 1-7; 91
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473425]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Lei, Yong-Min,Xiao, Meng-Meng,Li, Yu-Tao,et al. Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor[J]. BIOSENSORS & BIOELECTRONICS,2017.
APA	Lei, Yong-Min.,Xiao, Meng-Meng.,Li, Yu-Tao.,Xu, Li.,Zhang, Hong.,...&Zhang, Guo-Jun.(2017).Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor.BIOSENSORS & BIOELECTRONICS.
MLA	Lei, Yong-Min,et al."Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor".BIOSENSORS & BIOELECTRONICS (2017).