Regulation of cell arrangement using a novel composite micropattern

doi:10.1002/jbm.a.36157

CORC > 北京大学 > 信息科学技术学院

	Regulation of cell arrangement using a novel composite micropattern
	Liu, Xiaoyi ; Liu, Yaoping ; Zhao, Feng ; Hun, Tingting ; Li, Shan ; Wang, Yuguang ; Sun, Weijie ; Wang, Wei ; Sun, Yan ; Fan, Yubo
刊名	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
	2017
关键词	cell capture cell arrangement micropattern myoblasts actin alignment P38-ALPHA/BETA MAPK SKELETAL MYOBLASTS IN-VITRO DIFFERENTIATION ARRAYS ALIGNMENT FUSION MUSCLE CONTRACTILITY MYOGENESIS
DOI	10.1002/jbm.a.36157
英文摘要	Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 m in diameter for single-cell capture, surrounded by small, square arrays (3 m x 3 m) for cell spreading. This is surrounded by a border 2 m wide for restricting cell edges. The composite pattern results in two-cell and three-cell capture efficiencies of 32.1%+/- 1.94% and 24.2%+/- 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi-cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093-3101, 2017.; National Natural Science Foundation of China [31470942, 11421202, 61227902, 11072021]; National Key Research and Development Program [2016YFC1100704]; 111 Project [B13003]; SCI(E); ARTICLE; 11; 3093-3101; 105
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470427]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Liu, Xiaoyi,Liu, Yaoping,Zhao, Feng,et al. Regulation of cell arrangement using a novel composite micropattern[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2017.
APA	Liu, Xiaoyi.,Liu, Yaoping.,Zhao, Feng.,Hun, Tingting.,Li, Shan.,...&Fan, Yubo.(2017).Regulation of cell arrangement using a novel composite micropattern.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A.
MLA	Liu, Xiaoyi,et al."Regulation of cell arrangement using a novel composite micropattern".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A (2017).