A Flow-Through Cell Electroporation Device for Rapidly and Efficiently   Transfecting Massive Amounts of Cells in vitro and ex vivo

doi:10.1038/srep18469

CORC > 北京大学 > 信息科学技术学院

	A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo
	Zhao, Deyao ; Huang, Dong ; Li, Yang ; Wu, Mengxi ; Zhong, Wenfeng ; Cheng, Qiang ; Wang, Xiaoxia ; Wu, Yidi ; Zhou, Xiao ; Wei, Zewen ; Li, Zhihong ; Liang, Zicai
刊名	SCIENTIFIC REPORTS
	2016
关键词	LARGE-VOLUME MICROFLUIDIC ELECTROPORATION MAMMALIAN-CELLS DELIVERY MOLECULES LEUKEMIA THERAPY SYSTEM SCALE
DOI	10.1038/srep18469
英文摘要	Continuous cell electroporation is an appealing non-viral approach for genetically transfecting a large number of cells. Yet the traditional macro-scale devices suffer from the unsatisfactory transfection efficiency and/or cell viability due to their high voltage, while the emerging microfluidic electroporation devices is still limited by their low cell processing speed. Here we present a flowthrough cell electroporation device integrating large-sized flow tube and small-spaced distributed needle electrode array. Relatively large flow tube enables high flow rate, simple flow characterization and low shear force, while well-organized needle array electrodes produce an even-distributed electric field with low voltage. Thus the difficulties for seeking the fine balance between high flow rate and low electroporation voltage were steered clear. Efficient in vitro electrotransfection of plasmid DNA was demonstrated in several hard-to-transfect cell lines. Furthermore, we also explored ex vivo electroporated mouse erythrocyte as the carrier of RNA. The strong ability of RNA loading and short exposure time of freshly isolated cells jointly ensured a high yield of valid carrier erythrocytes, which further successfully delivered RNA into targeted tissue. Both in vitro and ex vivo electrotransfection could be accomplished at high cell processing speed (20 million cells per minute) which remarkably outperforms previous devices.; National Natural Science Foundation of China [61204118, 81473128, 81273422]; SCI(E); PubMed; ARTICLE; weizw@nanoctr.cn; zhhli@ime.pku.edu.cn; liangz@pku.edu.cn; 18469; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435508]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Zhao, Deyao,Huang, Dong,Li, Yang,et al. A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo[J]. SCIENTIFIC REPORTS,2016.
APA	Zhao, Deyao.,Huang, Dong.,Li, Yang.,Wu, Mengxi.,Zhong, Wenfeng.,...&Liang, Zicai.(2016).A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo.SCIENTIFIC REPORTS.
MLA	Zhao, Deyao,et al."A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo".SCIENTIFIC REPORTS (2016).